_EC 2.1.1.72 Site-specific DNA-methyltransferase (adenine-specific). 36 PDB entries  
EC 2.-.-.- Transferases. [19,022 PDB entries]
EC 2.1.-.- Transferring one-carbon groups. [1,783 PDB entries]
EC 2.1.1.- Methyltransferases. [1,448 PDB entries]
EC 2.1.1.72 Site-specific DNA-methyltransferase (adenine-specific). [36 PDB entries]    
1adm

Reaction: S-adenosyl-L-methionine + Dna adenine = S-adenosyl-L-homocysteine + Dna 6-methylaminopurine.
 


S-adenosyl-L-methionine
+
DNA adenine
=
S-adenosyl-L-homocysteine
+
DNA 6-methylaminopurine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Modification methylase. N-6 adenine-specific Dna methylase. Restriction-modification system.
Comments: This is a large group of enzymes, the majority of which, with enzymes of similar site specificity listed as Ec 3.1.21.3, Ec 3.1.21.4 and Ec 3.1.21.5, form so-called restriction-modification systems. See the Rebase database for a complete list of these enzymes: http://rebase.neb.com/rebase/
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 36 PDB entries in enzyme class E.C.2.1.1.72

  PDB code Protein
1adm
Adenine-n6-DNA-methyltransferase taqi. Chain: a, b. Ec: 2.1.1.72
Source: Thermus aquaticus
Chains: A, B (415 residues) CATH domains: 3.40.50.150 3.90.220.10
1aqi
Structure of adenine-n6-DNA-methyltransferase taqi
Source: Thermus aquaticus. Organism_taxid: 271. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (382 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1aqj
Structure of adenine-n6-DNA-methyltransferase taqi
Source: Thermus aquaticus. Organism_taxid: 271. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (381 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group SFG is 51.43% similar to enzyme product S-adenosylhomocysteine
1eg2
Crystal structure of rhodobacter spheroides (n6 adenosine) methyltransferase (m.Rsri)
Source: Rhodobacter sphaeroides. Organism_taxid: 1063.
Chain: A (270 residues) CATH domain: 3.40.50.150
Bound ligand:   Het Group MTA is 76.92% similar to enzyme product S-adenosylhomocysteine
1g38
Adenine-specific methyltransferase m. Taq i/DNA complex
Source: Synthetic: yes. Thermus aquaticus. Organism_taxid: 271. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, D (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
1g60
Crystal structure of methyltransferase mboiia (moraxella bovis)
Source: Moraxella bovis. Organism_taxid: 476. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (238 residues) CATH domain: 3.40.50.150
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
1nw5
Structure of the beta class n6-adenine DNA methyltransferase bound to s-adenosylmethionine
Source: Rhodobacter sphaeroides. Organism_taxid: 1063. Gene: rsrim. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (270 residues) CATH domain: 3.40.50.150
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
1nw6
Structure of the beta class n6-adenine DNA methyltransferase bound to sinefungin
Source: Rhodobacter sphaeroides. Organism_taxid: 1063. Gene: rsrim. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (271 residues) CATH domain: 3.40.50.150
Bound ligand:   Het Group SFG is 51.43% similar to enzyme product S-adenosylhomocysteine
1nw7
Structure of the beta class n6-adenine DNA methyltransferase bound to s-adenosyl-l-homocysteine
Source: Rhodobacter sphaeroides. Organism_taxid: 1063. Gene: rsrim. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (271 residues) CATH domain: 3.40.50.150
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1nw8
Structure of l72p mutant beta class n6-adenine DNA methyltra rsri
Source: Rhodobacter sphaeroides. Organism_taxid: 1063. Gene: rsrim. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (272 residues) CATH domain: 3.40.50.150
1q0s
Binary structure of t4dam with adohcy
Source: Enterobacteria phage t4. Organism_taxid: 10665. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (241 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1q0t
Ternary structure of t4dam with adohcy and DNA
Source: Synthetic: yes. Enterobacteria phage t4. Organism_taxid: 10665. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (241 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1yf3
T4dam in complex with adohcy and 13-mer oligonucleotide maki and semi-specific (~1/4) contact
Source: Synthetic: yes. Other_details: synthesized by new england biolabs. Enterobacteria phage t4. Organism_taxid: 10665. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (259 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1yfj
T4dam in complex with adohcy and 15-mer oligonucleotide showing semi-specific and specific contact
Source: Synthetic: yes. Other_details: synthesized by new england biolabs. Enterobacteria phage t4. Organism_taxid: 10665. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (259 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
1yfl
T4dam in complex with sinefungin and 16-mer oligonucleotide semi-specific and specific contact and flipped base
Source: Synthetic: yes. Other_details: synthesized by new england biolabs. Enterobacteria phage t4. Organism_taxid: 10665. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, D, E (246 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SFG is 51.43% similar to enzyme product S-adenosylhomocysteine
2adm
Adenine-n6-DNA-methyltransferase taqi
Source: Thermus aquaticus. Organism_taxid: 271. Gene: taq. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (385 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
2ar0
Crystal structure of type i restriction enzyme ecoki m protein (ec 2.1.1.72) (m.Ecoki)
Source: Escherichia coli. Organism_taxid: 562. Gene: hsdm, hsm. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (485 residues) CATH domains: Unassigned Unassigned
2dpm
Dpnm DNA adenine methyltransferase from streptoccocus pneumo complexed with s-adenosylmethionine
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: hb264. Gene: dpnm. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (258 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
2g1p
Structure of e. Coli DNA adenine methyltransferase (dam)
Source: Synthetic: yes. Escherichia coli. Organism_taxid: 562. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (244 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
2ibs
Crystal structure of the adenine-specific DNA methyltransferase m.Taqi complexed with the cofactor analog aeta and a 10 bp DNA containing 2-aminopurine at the target position
Source: Synthetic: yes. Other_details: solid-phase DNA synthesis using commercially available phosphoramidites. Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, D (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.00% similar to enzyme product S-adenosylhomocysteine
2ibt
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con aminopurine at the target position and an abasic site analo target base partner position
Source: Synthetic: yes. Other_details: solid-phase DNA synthesis using commercially available phosphoramidites. Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, D (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2ih2
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con methylpyrimidin-2(1h)-one at the target base partner positi
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: solid-phase DNA synthesis. Other_details: solid-phase DNA synthesis
Chains: A, D (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2ih4
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con pyrrolo-dc at the target base partner position
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: solid-phase DNA synthesis. Other_details: solid-phase DNA synthesis
Chains: A, D (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2ih5
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con abasic site analog at the target base partner position
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: solid-phase DNA synthesis. Other_details: solid-phase DNA synthesis
Chain: A (390 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2jg3
Mtaqi with baz
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Chains: A, D (392 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group BA2 is 58.06% similar to enzyme product S-adenosylhomocysteine
2np6
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con abasic site analog at the target position
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: solid-phase DNA synthesis. Other_details: solid-phase DNA synthesis
Chains: A, D (394 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2np7
Crystal structure of the adenine-specific DNA methyltransfer complexed with the cofactor analog aeta and a 10 bp DNA con abasic site analog at the target position and pyrrolo-dc at target base partner position
Source: Thermus aquaticus. Organism_taxid: 271. Strain: yt1. Gene: taqim. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: solid-phase DNA synthesis. Other_details: solid-phase DNA synthesis
Chain: A (393 residues) CATH domains: 3.40.50.150 3.90.220.10
Bound ligand:   Het Group NEA is 77.78% similar to enzyme product S-adenosylhomocysteine
2okc
Crystal structure of type i restriction enzyme stysji m prot (np_813429.1) from bacteroides thetaiotaomicron vpi-5482 at resolution
Source: Bacteroides thetaiotaomicron vpi-5482. Organism_taxid: 226186. Strain: vpi-5482, e50. Atcc: 29148. Gene: np_813429.1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (424 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
2ore
Binary structure of escherichia coli DNA adenine methyltransferase and s-adenosylhomocysteine
Source: Escherichia coli. Organism_taxid: 562. Gene: dam. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
2y7c
Atomic model of the ocr-bound methylase complex from the type i restriction-modification enzyme ecoki (m2s1). Based on fitting into em map 1534.
Source: Escherichia coli. Organism_taxid: 562. Strain: k. Enterobacteria phage t7. Organism_taxid: 10760. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: B, C (529 residues)
2y7h
Atomic model of the DNA-bound methylase complex from the type i restriction-modification enzyme ecoki (m2s1). Based on fitting into em map 1534.
Source: Escherichia coli. Organism_taxid: 562. Strain: k. Synthetic: yes. Organism_taxid: 562
Chains: B, C (529 residues)
Bound ligand:   Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine
4gbe
Crystal structure of e. Coli DNA adenine methyltransferase i with sah
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3387, dam, jw3350. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SAH corresponds to enzyme product S-adenosylhomocysteine
4gol
Crystal structure of e. Coli DNA adenine methyltransferase i with methylated aza-sam
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3387, dam, jw3350. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group SA8 is 51.43% similar to enzyme product S-adenosylhomocysteine
4gom
Crystal structure of e. Coli DNA adenine methyltransferase i with aza-sam
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3387, dam, jw3350. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group 0Y0 is 52.94% similar to enzyme product S-adenosylhomocysteine
4gon
Crystal structure of e. Coli DNA adenine methyltransferase i with indole aza-sam
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3387, dam, jw3350. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group 0Y1 is 40.00% similar to enzyme product S-adenosylhomocysteine
4goo
Crystal structure of e. Coli DNA adenine methyltransferase i with benzothiophene aza-sam
Source: Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: b3387, dam, jw3350. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: D, E, F (243 residues) CATH domains: 3.40.50.150 1.10.1020.10
Bound ligand:   Het Group 0Y2 is 40.00% similar to enzyme product S-adenosylhomocysteine