_EC 2.1.1.148 Thymidylate synthase (FAD). 34 PDB entries  
EC 2.-.-.- Transferases. [17,943 PDB entries]
EC 2.1.-.- Transferring one-carbon groups. [1,710 PDB entries]
EC 2.1.1.- Methyltransferases. [1,380 PDB entries]
EC 2.1.1.148 Thymidylate synthase (FAD). [34 PDB entries]    
1kq4

Reaction: 5,10-methylenetetrahydrofolate + dUMP + Nadph = dTMP + tetrahydrofolate + NADP(+).
 


5,10-methylenetetrahydrofolate
+
dUMP
+
NADPH
=
dTMP
+
tetrahydrofolate
+
NADP(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Fdts. Flavin dependent thymidylate synthase.
Comments: Contains Fad. All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from Nadph.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 34 PDB entries in enzyme class E.C.2.1.1.148

  PDB code Protein
1kq4
Crystal structure of a thy1-complementing protein (tm0449) f thermotoga maritima at 2.25 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (203 residues)
Bound ligand:   Het Group FAD corresponds to enzyme reactant FADH(2)
1o24
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima at 2.0 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (208 residues) CATH domain: Unassigned
1o25
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with dump at 2.4 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (214 residues) CATH domain: Unassigned
Bound ligand:   Het Group UMP corresponds to enzyme reactant dUMP
1o26
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fad and dump at 1.6 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (219 residues) CATH domain: Unassigned
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD corresponds to enzyme reactant FADH(2)
1o27
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fad and brdump at 2.3 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (216 residues) CATH domain: Unassigned
Bound ligands:   Het Group BRU is 95.00% similar to enzyme reactant dUMP
  Het Group FAD corresponds to enzyme reactant FADH(2)
1o28
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fdump at 2.1 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (218 residues) CATH domain: Unassigned
Bound ligand:   Het Group UFP is 95.00% similar to enzyme reactant dUMP
1o29
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fad and fdump at 2.0 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (216 residues) CATH domain: Unassigned
Bound ligands:   Het Group UFP is 95.00% similar to enzyme reactant dUMP
  Het Group FAD corresponds to enzyme reactant FADH(2)
1o2a
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fad at 1.8 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (211 residues) CATH domain: Unassigned
Bound ligand:   Het Group FAD corresponds to enzyme reactant FADH(2)
1o2b
Crystal structure of thymidylate synthase complementing protein (tm0449) from thermotoga maritima with fad and po4 at 2.45 a resolution
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: tm0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (213 residues) CATH domain: Unassigned
Bound ligand:   Het Group FAD corresponds to enzyme reactant FADH(2)
2af6
Crystal structure of mycobacterium tuberculosis flavin depen thymidylate synthase (mtb thyx) in the presence of co-facto substrate analog 5-bromo-2'-deoxyuridine-5'-monophosphate (
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: thyx. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H (245 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligands:   Het Group BRU is 95.24% similar to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
2cfa
Structure of viral flavin-dependant thymidylate synthase thy
Source: Paramecium bursaria chlorella virus 1. Organism_taxid: 10506. Expressed in: escherichia coli. Expression_system_taxid: 668369. Expression_system_variant: delta thya.
Chains: A, B (180 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group FAD is 71.19% similar to enzyme reactant NADPH
2gq2
Mycobacterium tuberculosis thyx-NADP complex
Source: Mycobacterium tuberculosis h37rv. Organism_taxid: 83332. Strain: h37rv. Gene: thyx, rv2754c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (242 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group NAP corresponds to enzyme reactant NADPH
3ah5
Crystal structure of flavin dependent thymidylate synthase t helicobacter pylori complexed with fad and dump
Source: Helicobacter pylori. Campylobacter pylori. Organism_taxid: 85962. Strain: 26695. Gene: hp_1533, thyx. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F (215 residues) CATH domain: Unassigned
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD corresponds to enzyme reactant FADH(2)
3fnn
Biochemical and structural analysis of an atypical thyx: corynebacterium glutamicum nchu 87078 depends on thya for thymidine biosynthesis
Source: Corynebacterium glutamicum. Brevibacterium flavum. Organism_taxid: 1718. Strain: 87078. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (227 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group FAD corresponds to enzyme reactant FADH(2)
3g4a
Crystal structure of flavine dependant thymidylate synthase mutant from thermotoga maritima at 1.95 angstrom resolution
Source: Thermotoga maritima msb8. Organism_taxid: 243274. Strain: msb8 / dsm 3109 / jcm 10099. Atcc: 43589. Gene: thy1, thyx, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (218 residues)
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
3g4c
Flavine dependant thymidylate syntahse s88c mutant
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: thy1, thyx, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (209 residues)
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
3ge9
A structurally atypical thyx from corynebacterium glutamicum nchu 87078 is not required for thymidylate biosynthesis
Source: Corynebacterium glutamicum. Organism_taxid: 1718. Strain: 87078. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (216 residues) CATH domains: Unassigned Unassigned Unassigned
3gwc
Crystal structure of mycobacterium tuberculosis thymidylate bound to fdump and fad
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: thyx, rv2754c, mt2824, mtv002.19c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D, E, F, G, H (246 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligands:   Het Group UFP is 95.24% similar to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
3hzg
Crystal structure of mycobacterium tuberculosis thymidylate bound with fad
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: thyx, rv2754c, mt2824, mtv002.19c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (245 residues) CATH domains: Unassigned Unassigned Unassigned
Bound ligand:   Het Group FAD is 71.19% similar to enzyme reactant NADPH
3n0b
Tm0449 mutant crystals grown in loops/micromounts
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (216 residues) CATH domain: Unassigned
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
3n0c
Tm0449 mutant crystal grown by hanging drop method
Source: Thermotoga maritima. Organism_taxid: 2336. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (216 residues) CATH domain: Unassigned
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
3n3y
Crystal structure of thymidylate synthase x (thyx) from heli pylori with fad and dump at 2.31a resolution
Source: Helicobacter pylori. Organism_taxid: 102617. Strain: ss1. Gene: thyx. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D (209 residues) CATH domain: Unassigned
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD corresponds to enzyme reactant FADH(2)
4fzb
Structure of thymidylate synthase thyx complexed to a new in
Source: Paramecium bursaria chlorella virus 1. Pbcv-1. Organism_taxid: 10506. Strain: paramecium bursaria chlorella virus (pbcv-1). Gene: a674r, thyx. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P (191 residues) CATH domains: Unassigned Unassigned
Bound ligand:   Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gt9
T. Maritima fdts with fad, dump and folate.
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (214 residues)
Bound ligands:   Het Group MEF is 72.73% similar to enzyme reactant 5,10-methylenetetrahydrofolate
  Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gta
T. Maritima fdts with fad, dump, and folinic acid
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (215 residues)
Bound ligands:   Het Group FFO is 70.59% similar to enzyme reactant 5,10-methylenetetrahydrofolate
  Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gtb
T. Maritima fdts with fad, dump, and raltitrexed.
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (211 residues)
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gtc
T. Maritima fdts (e144r mutant) plus fad
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (218 residues)
Bound ligand:   Het Group FAD is 72.92% similar to enzyme reactant NADPH
4gtd
T. Maritima fdts (e144r mutant) with fad and dump
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (210 residues)
Bound ligands:   Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gte
T. Maritima fdts (e144r mutant) with fad and folate
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (215 residues)
Bound ligands:   Het Group FAD is 72.92% similar to enzyme reactant NADPH
  Het Group MEF is 46.88% similar to enzyme product 5,6,7,8-tetrahydrofolate
4gtf
T. Maritima fdts (h53a mutant) with fad, dump and folate
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (213 residues)
Bound ligands:   Het Group MEF is 72.73% similar to enzyme reactant 5,10-methylenetetrahydrofolate
  Het Group UMP corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4gtl
T. Maritima fdts (r174k mutant) with fad
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thyx, thy1, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (216 residues)
Bound ligand:   Het Group FAD is 71.19% similar to enzyme reactant NADPH
4kar
Crystal structure of fdts (tm0449) mutant (h53d) with fad
Source: Thermotoga maritima msb8. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (214 residues) CATH domain: Unassigned
Bound ligand:   Het Group FAD is 72.92% similar to enzyme reactant NADPH
4kas
Crystal structure of fdts from t. Maritima mutant (h53d) wit dump
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (213 residues) CATH domain: Unassigned
Bound ligands:   Het Group DU corresponds to enzyme reactant dUMP
  Het Group FAD is 71.19% similar to enzyme reactant NADPH
4kat
Crystal structure of fdts from t. Maritima mutant (r174k) wi dump
Source: Thermotoga maritima msb8. Organism_taxid: 243274. Strain: atcc 43589 / msb8 / dsm 3109 / jcm 10099. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (218 residues) CATH domain: Unassigned
Bound ligands:   Het Group DU corresponds to enzyme reactant dUMP
  Het Group FDA is 71.19% similar to enzyme reactant NADPH