_EC 1.5.99.12 Cytokinin dehydrogenase. 16 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,653 PDB entries]
EC 1.5.-.- Acting on the CH-NH group of donors. [619 PDB entries]
EC 1.5.99.- With other acceptors. [19 PDB entries]
EC 1.5.99.12 Cytokinin dehydrogenase. [16 PDB entries]    
1w1o

Reaction: N(6)-dimethylallyladenine + acceptor + H(2)O = adenine + 3-methylbut-2- enal + reduced acceptor.
 


N(6)-dimethylallyladenine
+ acceptor
+ H(2)O
=
adenine
+
3-methylbut-2- enal
+ reduced acceptor
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Cytokinin oxidase.
Cofactor(s): Fad.
 

FAD
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Converts zeatin and related cytokinins. Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalyzed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(2+) in the presence of imidazole can act as acceptors.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 16 PDB entries in enzyme class E.C.1.5.99.12

  PDB code Protein
1w1o
Native cytokinin dehydrogenase
Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
Chain: A (479 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
1w1q
Plant cytokinin dehydrogenase in complex with isopentenyladenine
Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
Chain: A (479 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZIP corresponds to enzyme reactant N(6)-dimethylallyladenine
  Het Group FAD corresponds to enzyme cofactor FAD
1w1r
Plant cytokinin dehydrogenase in complex with trans-zeatin
Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
Chain: A (479 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZEA is 93.00% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group FAD corresponds to enzyme cofactor FAD
1w1s
Plant cytokinin dehydrogenase in complex with benzylaminopurine
Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
Chain: A (479 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group EMU is 77.00% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group FAD corresponds to enzyme cofactor FAD
2exr
X-ray structure of cytokinin oxidase/dehydrogenase (ckx) fro arabidopsis thaliana at5g21482
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: ckx7, ckx5. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (491 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
2q4w
Ensemble refinement of the protein crystal structure of cyto oxidase/dehydrogenase (ckx) from arabidopsis thaliana at5g2
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Strain: cv. Columbia. Gene: ckx7, ckx5. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (491 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
2qkn
Crystal structure of maize cytokinin oxidase/dehydrogenasE C with phenylurea inhibitor cppu
Source: Zea mays. Maize. Organism_taxid: 4577. Variant: nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (496 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
2qpm
Leu492ala mutant of maize cytokinin oxidase/dehydrogenase co with benzylurea inhibitor cpbu
Source: Zea mays. Maize. Organism_taxid: 4577. Variant: nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (496 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group 246 is 40.00% similar to enzyme product adenine
  Het Group FAD corresponds to enzyme cofactor FAD
3bw7
Maize cytokinin oxidase/dehydrogenase complexed with the all cytokinin analog ha-1
Source: Zea mays. Maize. Gene: ckx1, zmcko1. Expressed in: yarrowia lipolytica.
Chain: A (497 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group 301 is 87.50% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3c0p
Maize cytokinin oxidase/dehydrogenase complexed with the all cytokinin analog ha-8
Source: Zea mays. Maize. Organism_taxid: 4577. Gene: ckx1, zmcko1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (496 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group HA8 is 93.33% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3dq0
Maize cytokinin oxidase/dehydrogenase complexed with n6-(3-m phenyl)adenine
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (498 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group MZO is 73.68% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group FAD corresponds to enzyme cofactor FAD
3kjm
Leu492ala mutant of maize cytokinin oxidase/dehydrogenase co with phenylurea inhibitor cppu
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: cko1/ckx1, ckx1. Expressed in: yarrowia lipolytica clib122. Expression_system_taxid: 284591.
Chain: A (496 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3s1c
Maize cytokinin oxidase/dehydrogenase complexed with n6- isopentenyladenosine
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (498 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZIR is 62.50% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3s1d
Glu381ser mutant of maize cytokinin oxidase/dehydrogenase co with n6-isopentenyladenosine
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (498 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZIR is 62.50% similar to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3s1e
Pro427gln mutant of maize cytokinin oxidase/dehydrogenase co with n6-isopentenyladenine
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (499 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZIP corresponds to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD
3s1f
Asp169glu mutant of maize cytokinin oxidase/dehydrogenase co with n6-isopentenyladenine
Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
Chain: A (498 residues) CATH domains: Unassigned 3.30.465.10 3.40.462.10
Bound ligands:   Het Group ZIP corresponds to enzyme reactant N(6)-dimethylallyladenine
  Het Group GOL is 50.00% similar to enzyme product 3-methylbut-2-
  Het Group FAD corresponds to enzyme cofactor FAD