| Reaction: |
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N(6)-dimethylallyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-
enal + reduced acceptor.
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N(6)-dimethylallyladenine
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+
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acceptor
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+
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H(2)O
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=
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adenine
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+
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3-methylbut-2-enal
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+
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reduced acceptor
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Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
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| Other name(s): |
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Cytokinin oxidase.
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| Cofactor(s): |
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Fad.
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FAD
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Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
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| Comments: |
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Converts zeatin and related cytokinins.
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
a dimethylallyl or other prenyl group.
Although this activity was previously thought to be catalyzed by a
hydrogen-peroxide-forming oxidase, this enzyme does not require
oxygen for activity and does not form hydrogen peroxide.
2,6-dichloroindophenol, methylene blue, nitroblue tetrazolium,
phenazine methosulfate and Cu(2+) in the presence of imidazole can
act as acceptors.
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| Links: |
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PDB code
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Protein
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Native cytokinin dehydrogenase
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Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
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Chain:
A
(479 residues)
CATH domains:
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Plant cytokinin dehydrogenase in complex with isopentenyladenine
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Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
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Chain:
A
(479 residues)
CATH domains:
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Bound ligand:
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Het Group ZIP
corresponds to
enzyme reactant N(6)-dimethylallyladenine
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Plant cytokinin dehydrogenase in complex with trans-zeatin
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Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
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Chain:
A
(479 residues)
CATH domains:
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Bound ligand:
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Het Group ZEA
is 93.00% similar to
enzyme reactant N(6)-dimethylallyladenine
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Plant cytokinin dehydrogenase in complex with benzylaminopurine
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Source: Zea mays. Maize. Organism_taxid: 4577. Expressed in: pichia pastoris. Expression_system_taxid: 4922
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Chain:
A
(479 residues)
CATH domains:
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Bound ligand:
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Het Group EMU
is 77.00% similar to
enzyme reactant N(6)-dimethylallyladenine
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X-ray structure of cytokinin oxidase/dehydrogenase (ckx) from arabidopsis thaliana at5g21482
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Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: ckx7, ckx5. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Chain:
A
(491 residues)
CATH domains:
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Ensemble refinement of the protein crystal structure of cytokinin oxidase/dehydrogenase (ckx) from arabidopsis thaliana at5g21482
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Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Strain: cv. Columbia. Gene: ckx7, ckx5. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Chain:
A
(491 residues)
CATH domains:
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Crystal structure of maize cytokinin oxidase/dehydrogenase complexed with phenylurea inhibitor cppu
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Source: Zea mays. Maize. Organism_taxid: 4577. Variant: nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
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Chain:
A
(496 residues)
CATH domains:
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Leu492ala mutant of maize cytokinin oxidase/dehydrogenase complexed with benzylurea inhibitor cpbu
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Source: Zea mays. Maize. Organism_taxid: 4577. Variant: nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
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Chain:
A
(496 residues)
CATH domains:
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Maize cytokinin oxidase/dehydrogenase complexed with the allenic cytokinin analog ha-1
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Source: Zea mays. Maize. Gene: ckx1, zmcko1. Expressed in: yarrowia lipolytica.
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Chain:
A
(497 residues)
CATH domains:
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Maize cytokinin oxidase/dehydrogenase complexed with the allenic cytokinin analog ha-8
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Source: Zea mays. Maize. Gene: ckx1, zmcko1. Expressed in: yarrowia lipolytica.
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Chain:
A
(496 residues)
CATH domains:
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Maize cytokinin oxidase/dehydrogenase complexed with n6-(3- methoxy-phenyl)adenine
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Source: Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.
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Chain:
A
(498 residues)
CATH domains:
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