_EC 1.2.1.27 Methylmalonate-semialdehyde dehydrogenase (CoA acylating). 3 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,653 PDB entries]
EC 1.2.-.- Acting on the aldehyde or oxo group of donors. [500 PDB entries]
EC 1.2.1.- With NAD(+) or NADP(+) as acceptor. [369 PDB entries]
EC 1.2.1.27 Methylmalonate-semialdehyde dehydrogenase (CoA acylating). [3 PDB entries]    
1t90

Reaction: 2-methyl-3-oxopropanoate + CoA + H(2)O + NAD(+) = propanoyl-CoA + HCO(3)(-) + Nadh.
 


2-methyl-3-oxopropanoate
+
CoA
+ H(2)O
+
NAD(+)
=
propanoyl-CoA
+
HCO(3)(-)
+
NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Methylmalonate-semialdehyde dehydrogenase (acylating). Mmsa dehydrogenase. Msdh.
Comments: Also converts 3-oxopropanoate into acetyl-CoA. The reaction occurs in two steps with the decarboxylation process preceding CoA-binding. Bicarbonate rather than CO(2) is released as a final product.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 3 PDB entries in enzyme class E.C.1.2.1.27

  PDB code Protein
1t90
Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: mmsa, iola, e83a, bsu39760. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (484 residues) CATH domains: 3.40.605.10 3.40.309.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
4e4g
Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021
Source: Sinorhizobium meliloti. Organism_taxid: 266834. Strain: 1021. Gene: iola, mmsa, r00726, smc00781. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F, G, H (488 residues) CATH domains: 3.40.605.10 3.40.309.10
4iym
Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed wi target 011934
Source: Sinorhizobium meliloti. Ensifer meliloti. Organism_taxid: 266834. Strain: 1021. Gene: iola, mmsa, r00726, smc00781. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P (488 residues) CATH domains: 3.40.605.10 3.40.309.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)