_EC 1.18.6.1 Nitrogenase. 35 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,663 PDB entries]
EC 1.18.-.- Acting on iron-sulfur proteins as donors. [121 PDB entries]
EC 1.18.6.- With dinitrogen as acceptor. [35 PDB entries]
EC 1.18.6.1 Nitrogenase. [35 PDB entries]    
1cp2

Pathway: Nitrogenase
Reaction: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 Atp + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 Adp + 16 phosphate.
 

8 × reduced ferredoxin
+ 8 × H(+)
+
N(2)
+
16 × ATP
+ 16 × H(2)O
= 8 × oxidized ferredoxin
+ H(2)
+ 2 × NH(3)
+
16 × ADP
+
16 × phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Cofactor(s): Iron-sulfur; Vanadium cation or Mo cation.
 

Iron-sulfur
Vanadium cation
or Mo cation
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Composed of two proteins that can be separated but are both required for nitrogenase activity. Dinitrogen reductase is a [4Fe-4S] protein, which, with two molecules of Atp and ferredoxin, generates an electron. The electron is transferred to the other protein, dinitrogenase (molybdoferredoxin). Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen in three succesive two-electron reductions from nitrogen to diimine to hydrazine to two molecules of ammonia; the molybdenum may be replaced by vanadium or iron.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 35 PDB entries in enzyme class E.C.1.18.6.1

  PDB code Protein
1cp2
Nitrogenase iron protein from clostridium pasteurianum
Source: Clostridium pasteurianum. Organism_taxid: 1501. Cell_line: w5. Atcc: atcc 6013. Collection: atcc 6013
Chains: A, B (269 residues) CATH domain: 3.40.50.300
1de0
Modulating the midpoint potential of the [4fe-4s] cluster of the nitrogenase fe protein
Source: Azotobacter vinelandii. Organism_taxid: 354
Chains: A, B (289 residues) CATH domain: 3.40.50.300
1fp4
Crystal structure of the alpha-h195q mutant of nitrogenase
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D (467 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1fp6
The nitrogenase fe protein from azotobacter vinelandii complexed with mgadp
Source: Azotobacter vinelandii. Organism_taxid: 354. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B, C, D (289 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1g1m
All-ferrous nitrogenase iron protein from azotobacter vinelandii
Source: Azotobacter vinelandii. Organism_taxid: 354. Cell_line: nifh
Chains: A, B (287 residues) CATH domain: 3.40.50.300
1g20
Mgatp-bound and nucleotide-free structures of a nitrogenase complex between leu127del-fe protein and the mofe protein
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H (476 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1g21
Mgatp-bound and nucleotide-free structures of a nitrogenase protein complex between leu127del-fe protein and the mofe protein
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H (476 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
1g5p
Nitrogenase iron protein from azotobacter vinelandii
Source: Azotobacter vinelandii. Organism_taxid: 354. Strain: op. Other_details: gene: nifh
Chains: A, B (286 residues) CATH domain: 3.40.50.300
1h1l
Nitrogenase mo-fe protein from klebsiella pneumoniae, nifv mutant
Source: Klebsiella pneumoniae. Organism_taxid: 573. Strain: unf1613. Variant: nifv mutant. Variant: nifv mutant
Chains: A, B, C, D (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1l5h
Femo-cofactor deficient nitrogenase mofe protein
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B (405 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1lfx
Theoretical structure of nitrogenase molybdenum-iron protein alpha chain
Source: Rhizobium sp. Cowpea. Bacteria. Strain: irc78
Chain: A (500 residues)
1m1n
Nitrogenase mofe protein from azotobacter vinelandii
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H (477 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1m1y
Chemical crosslink of nitrogenase mofe protein and fe protein
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P (477 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1m34
Nitrogenase complex from azotobacter vinelandii stabilized b tetrafluoroaluminate
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1mio
X-ray crystal structure of the nitrogenase molybdenum-iron protein from clostridium pasteurianum at 3.0 angstroms resolution
Source: Clostridium pasteurianum. Organism_taxid: 1501. Organism_taxid: 1501
Chains: A, B, C, D (525 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1n2c
Nitrogenase complex from azotobacter vinelandii stabilized by adp-tetrafluoroaluminate
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1nip
Crystallographic structure of the nitrogenase iron protein from azotobacter vinelandii
Source: Azotobacter vinelandii. Organism_taxid: 354
Chains: A, B (283 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1qgu
Nitrogenase mo-fe protein from klebsiella pneumoniae, dithionite-reduced state
Source: Klebsiella pneumoniae. Organism_taxid: 573. Strain: m5a1. Variant: wild-type. Cellular_location: cytoplasm. Other_details: also known as klebsiella pneumoniae oxytoca. Other_details: also known as klebsiella pneumoniae oxytoca
Chains: A, B, C, D (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1qh1
Nitrogenase mofe protein from klebsiella pneumoniae, phenosafranin oxidized state
Source: Klebsiella pneumoniae. Organism_taxid: 573. Strain: m5a1. Variant: wild-type. Cellular_location: cytoplasm. Other_details: also known as klebsiella pneumoniae oxytoca.. Other_details: also known as klebsiella pneumoniae oxytoca.
Chains: A, B, C, D (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1qh8
Nitrogenase mofe protein from klebsiella pneumoniae, as- crystallized (mixed oxidation) state
Source: Klebsiella pneumoniae. Organism_taxid: 573. Strain: m5a1. Variant: wild-type. Cellular_location: cytoplasm. Other_details: also known as klebsiella pneumoniae oxytoca.. Other_details: also known as klebsiella pneumoniae oxytoca.
Chains: A, B, C, D (478 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
1rw4
Nitrogenase fe protein l127 deletion variant
Source: Azotobacter vinelandii. Organism_taxid: 354. Gene: nifh. Expressed in: azotobacter vinelandii. Expression_system_taxid: 354.
Chain: A (271 residues) CATH domain: 3.40.50.300
1xcp
Crystal structure of the nitrogenase fe protein phe135trp with mgadp bound
Source: Azotobacter vinelandii. Organism_taxid: 354. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (289 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1xd8
Crystal structure of the nitrogenase fe protein asp39asn
Source: Azotobacter vinelandii. Organism_taxid: 354. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (289 residues) CATH domain: 3.40.50.300
1xd9
Crystal structure of the nitrogenase fe protein asp39asn with mgadp bound
Source: Azotobacter vinelandii. Organism_taxid: 354. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (289 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
1xdb
Crystal structure of the nitrogenase fe protein asp129glu
Source: Azotobacter vinelandii. Organism_taxid: 354. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (289 residues) CATH domain: 3.40.50.300
2afh
Crystal structure of nucleotide-free av2-av1 complex
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F (476 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
2afi
Crystal structure of mgadp bound av2-av1 complex
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P (476 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
Bound ligand:   Het Group ADP corresponds to enzyme product ADP
2afk
Crystal structure of mgamppcp-bound av2-av1 complex
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D, E, F, G, H (477 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
Bound ligand:   Het Group ACP is 81.00% similar to enzyme product ADP
2c8v
Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: structural characterization of the nitrogenase fe protein leu127 deletion variant with bound mgatp
Source: Azotobacter vinelandii. Organism_taxid: 354
Chain: A (272 residues) CATH domain: 3.40.50.300
Bound ligand:   Het Group ATP corresponds to enzyme reactant ATP
2min
Nitrogenase mofe protein from azotobacter vinelandii, oxidized state
Source: Azotobacter vinelandii. Organism_taxid: 354. Strain: wild-type. Strain: wild-type
Chains: A, B, C, D (467 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
2nip
Nitrogenase iron protein from azotobacter vinelandii
Source: Azotobacter vinelandii. Organism_taxid: 354. Strain: op. Atcc: atcc 13705. Collection: atcc 13705
Chains: A, B (286 residues) CATH domain: 3.40.50.300
3k1a
Insights into substrate binding at femo-cofactor in nitrogenase from the structure of an alpha-70ile mofe protein variant
Source: Azotobacter vinelandii. Organism_taxid: 354. Gene: nifdk, nifk. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: nifd. Expression_system_taxid: 562
Chains: A, B, C, D (467 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
3min
Nitrogenase mofe protein from azotobacter vinelandii, oxidized state
Source: Azotobacter vinelandii. Organism_taxid: 354. Strain: wild-type. Strain: wild-type
Chains: A, B, C, D (467 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
3u7q
A. Vinelandii nitrogenase mofe protein at atomic resolution
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D (477 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980
4nd8
Av nitrogenase mofe protein high ph form
Source: Azotobacter vinelandii. Organism_taxid: 354. Organism_taxid: 354
Chains: A, B, C, D (477 residues) CATH domains: 3.40.50.1980 3.40.50.1980 3.40.50.1980