_EC 1.14.17.3 Peptidylglycine monooxygenase. 21 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,663 PDB entries]
EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [1,436 PDB entries]
EC 1.14.17.- With reduced ascorbate as one donor, and incorporation of one ato [23 PDB entries]
EC 1.14.17.3 Peptidylglycine monooxygenase. [21 PDB entries]    
1opm

Reaction: Peptidylglycine + ascorbate + O(2) = peptidyl(2-hydroxyglycine) + dehydroascorbate + H(2)O.
 


Peptidylglycine
+
ascorbate
+
O(2)
=
peptidyl(2-hydroxyglycine)
+
dehydroascorbate
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Pam. Peptidyl alpha-amidating enzyme. Peptidylglycine 2-hydroxylase. Peptidylglycine alpha-amidating monooxygenase.
Cofactor(s): Cu cation.
Comments: Peptidylglycines with a neutral amino acid residue in the penultimate position are the best substrates for the enzyme. The product is unstable and dismutates to glyoxylate and the corresponding desglycine peptide amide, a reaction catalyzed by Ec 4.3.2.5. Involved in the final step of biosynthesis of alpha-melanotropin and related biologically active peptides.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 21 PDB entries in enzyme class E.C.1.14.17.3

  PDB code Protein
1opm
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound substrate
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Cell_line: dg44. Organ: ovary. Organelle: secretory vesicles. Cellular_location: excreted. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (310 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligands:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
  Het Group IYG is 40.00% similar to enzyme product peptidyl(2-hydroxyglycine)
1phm
Peptidylglycine alpha-hydroxylating monooxygenase (phm) from
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Cell_line: dg44. Organ: pituitary. Organelle: secretory granule. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: dg44.
Chain: A (305 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
1sdw
Reduced (cu+) peptidylglycine alpha-hydroxylating monooxygen bound peptide and dioxygen
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: chinese hamster ovary (cho). Expression_system_organ: ovary.
Chain: A (312 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligands:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
  Het Group OXY corresponds to enzyme reactant O(2)
1yi9
Crystal structure analysis of the oxidized form of the m314i peptidylglycine alpha-hydroxylating monooxygenase
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (295 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
1yip
Oxidized peptidylglycine alpha-hydroxylating monooxygenase (phm) in a new crystal form
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (311 residues) CATH domains: 2.60.120.310 2.60.120.230
1yjk
Reduced peptidylglycine alpha-hydroxylating monooxygenase (p new crystal form
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (303 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
1yjl
Reduced peptidylglycine alpha-hydroxylating monooxygenase in a new crystal form
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (285 residues) CATH domains: 2.60.120.310 2.60.120.230
3fvz
Structure of peptidyl-alpha-hydroxyglycine alpha-amidating l
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: ovary cells
Chain: A (329 residues) CATH domain: 2.120.10.30
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3fw0
Structure of peptidyl-alpha-hydroxyglycine alpha-amidating lyase (pal) bound to alpha-hydroxyhippuric acid (non- peptidic substrate)
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: ovary cells
Chain: A (329 residues) CATH domain: 2.120.10.30
3mib
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound nitrite
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (308 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mic
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound azide obtained by co-crystallization
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (309 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mid
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound azide obtained by soaking (100mm nan3)
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (311 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mie
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound azide obtained by soaking (50mm nan3)
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (310 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mif
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound carbon monooxide (co)
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (310 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mig
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound nitrite, obtained in the presence of subst
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (309 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mih
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) with bound azide, obtained in the presence of substra
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (307 residues) CATH domains: 2.60.120.310 2.60.120.230
3mlj
Reduced (cu+) peptidylglycine alpha-hydroxylating monooxygen with bound carbon monooxide (co)
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (310 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
3mlk
Reduced (cu+) peptidylglycine alpha-hydroxylating monooxygen with bound nitrite
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (306 residues) CATH domains: 2.60.120.310 2.60.120.230
3mll
Reduced (cu+) peptidylglycine alpha-hydroxylating monooxygen with bound azide
Source: Rattus norvegicus. Rat. Organism_taxid: 10116. Strain: sprague-dawley. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (304 residues) CATH domains: 2.60.120.310 2.60.120.230
3phm
Reduced (cu+) peptidylglycine alpha-hydroxylating monooxygen
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Cell_line: dg44. Organ: ovary. Organelle: secretory vesicles. Cellular_location: excreted. Expressed in: cricetulus griseus. Expression_system_taxid: 10029.
Chain: A (310 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligand:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
4e4z
Oxidized (cu2+) peptidylglycine alpha-hydroxylating monooxyg (phm) in complex with hydrogen peroxide (1.98 a)
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: pam. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
Chain: A (312 residues) CATH domains: 2.60.120.310 2.60.120.230
Bound ligands:   Het Group GOL is 50.00% similar to enzyme reactant L-ascorbate
  Het Group PEO corresponds to enzyme reactant O(2)