_EC 1.14.15.6 Cholesterol monooxygenase (side-chain-cleaving). 6 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,840 PDB entries]
EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [1,477 PDB entries]
EC 1.14.15.- With a reduced iron-sulfur protein as one donor, and incorporatio [131 PDB entries]
EC 1.14.15.6 Cholesterol monooxygenase (side-chain-cleaving). [6 PDB entries]    
2asa

Reaction: Cholesterol + 6 reduced adrenodoxin + 3 O(2) = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H(2)O.
 


Cholesterol
+ 6 × reduced adrenodoxin
+
3 × O(2)
=
pregnenolone
+
4-methylpentanal
+ 6 × oxidized adrenodoxin
+ 4 × H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Cholesterol 20-22-desmolase. Cholesterol C(20-22) desmolase. Cholesterol desmolase. Cholesterol side-chain cleavage enzyme. Cholesterol side-chain-cleaving enzyme. CYP11A1. Cytochrome P-450(scc). Cytochrome p450(scc). Steroid 20-22 desmolase. Steroid 20-22-lyase.
Cofactor(s): Heme-thiolate.
Comments: The reaction proceeds in three stages, with two hydroxylations at C-22 and C-20 preceding scission of the side-chain between carbons 20 and 22. The initial source of the electrons is Nadph, which transfers the electrons to the adrenodoxin via Ec 1.18.1.6.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 6 PDB entries in enzyme class E.C.1.14.15.6

  PDB code Protein
2asa
Homology modeling of cytochrome p450scc
Source: Bos taurus. Bovine
Chain: A (430 residues)
Bound ligand:   Het Group HEM is 61.00% similar to enzyme reactant Cholesterol
3mzs
Crystal structure of cytochrome p450 cyp11a1 in complex with hydroxy-cholesterol
Source: Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Gene: cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (470 residues) CATH domain: 1.10.630.10
Bound ligand:   Het Group HC9 is 96.55% similar to enzyme reactant Cholesterol
3n9y
Crystal structure of human cyp11a1 in complex with cholester
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cyp11a, cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: adrenodoxin, adx, fdx1.
Chains: A, B (471 residues) CATH domain: 1.10.630.10
Bound ligand:   Het Group CLR corresponds to enzyme reactant Cholesterol
3n9z
Crystal structure of human cyp11a1 in complex with 22- hydroxycholesterol
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cyp11a, cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: adrenodoxin, adx, fdx1.
Chains: A, B (470 residues) CATH domain: 1.10.630.10
Bound ligand:   Het Group HC9 is 96.55% similar to enzyme reactant Cholesterol
3na0
Crystal structure of human cyp11a1 in complex with 20,22- dihydroxycholesterol
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cyp11a, cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: adrenodoxin, adx, fdx1.
Chains: A, B (470 residues) CATH domain: 1.10.630.10
Bound ligand:   Het Group 2DC is 93.33% similar to enzyme reactant Cholesterol
3na1
Crystal structure of human cyp11a1 in complex with 20- hydroxycholesterol
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cyp11a, cyp11a1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: adrenodoxin, adx, fdx1.
Chains: A, B (472 residues) CATH domain: 1.10.630.10
Bound ligand:   Het Group HCD is 96.55% similar to enzyme reactant Cholesterol