_EC 1.14.13.72 Methylsterol monooxygenase. 0 PDB entries  
EC 1.-.-.- Oxidoreductases. [9,371 PDB entries]
EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [1,555 PDB entries]
EC 1.14.13.- With Nadh or nadph as one donor, and incorporation of one atom of [735 PDB entries]
EC 1.14.13.72 Methylsterol monooxygenase. [-]    

Pathway: Sterol ring A modification
Reaction: (1) 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O. (2) 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4- alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O. (3) 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha- carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha- cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O.
 


4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol
+
NAD(P)H
+
O(2)
=
4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol
+
NAD(P)(+)
+ H(2)O

4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol
+
NAD(P)H
+
O(2)
=
3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4- alpha-carbaldehyde
+
NAD(P)(+)
+ 2 × H(2)O

3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha- carbaldehyde
+
NAD(P)H
+
O(2)
=
3-beta-hydroxy-4-beta-methyl-5-alpha- cholest-7-ene-4-alpha-carboxylate
+
NAD(P)(+)
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): 4-methylsterol oxidase. Methylsterol hydroxylase.
Comments: Requires cytochrome b5. Also acts on 4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol. The sterol can be based on cycloartenol as well as lanosterol. Formerly Ec 1.14.99.16.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are no PDB entries in enzyme class E.C.1.14.13.72