_EC 1.14.13.25 Methane monooxygenase (soluble). 32 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,621 PDB entries]
EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [1,435 PDB entries]
EC 1.14.13.- With Nadh or nadph as one donor, and incorporation of one atom of [659 PDB entries]
EC 1.14.13.25 Methane monooxygenase (soluble). [32 PDB entries]    
1fyz

Reaction: Methane + NAD(P)H + O(2) = methanol + NAD(P)(+) + H(2)O.
 

Methane
+
NAD(P)H
+
O(2)
=
methanol
+
NAD(P)(+)
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Methane hydroxylase.
Comments: The enzyme is soluble, in contrast to the particulate enzyme, Ec 1.14.18.3. Broad specificity; many alkanes can be hydroxylated, and alkenes are converted into the corresponding epoxides; Co is oxidized to CO(2), ammonia is oxidized to hydroxylamine, and some aromatic compounds and cyclic alkanes can also be hydroxylated, but more slowly.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 32 PDB entries in enzyme class E.C.1.14.13.25

  PDB code Protein
1fyz
Methane monooxygenase hydroxylase, form ii reduced by soaking
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fz0
Methane monooxygenase hydroxylase, form ii mixed-valent grown anaerobically
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fz1
Methane monooxygenase hydroxylase, form iii oxidized
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group FMT is 66.00% similar to enzyme product Methanol
1fz2
Methane monooxygenase hydroxylase, form ii mixed-valent generated by crystal soaking
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fz3
Methane monooxygenase hydroxylase, form iii soak at ph 6.2 (0.1 m pipes)
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group FMT is 66.00% similar to enzyme product Methanol
1fz4
Methane monooxygenase hydroxylase, form iii soaked at ph 8.5 (0.1 m tris)
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group FMT is 66.00% similar to enzyme product Methanol
1fz5
Methane monooxygenase hydroxylase, form ii crystallized anaerobically from reduced enzyme
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fz6
Methane monooxygenase hydroxylase, form ii soaked in 1 m methanol
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group MOH corresponds to enzyme product Methanol
1fz7
Methane monooxygenase hydroxylase, form iii soaked in 0.9 m ethanol
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group FMT is 66.00% similar to enzyme product Methanol
1fz8
Methane monooxygenase hydroxylase, form ii cocrystallized with dibromomethane
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fz9
Methane monooxygenase hydroxylase, form ii cocrystallized with iodoethane
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fzh
Methane monooxygenase hydroxylase, form ii pressurized with xenon gas
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
1fzi
Methane monooxygenase hydroxylase, form i pressurized with xenon gas
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (512 residues) CATH domain: 1.10.620.20
1jq4
[2fe-2s] domain of methane monooxygenase reductase from methylococcus capsulatus (bath)
Source: Methylococcus capsulatus str. Bath. Organism_taxid: 243233. Strain: bath. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (98 residues) CATH domain: 3.10.20.30
1mhy
Methane monooxygenase hydroxylase
Source: Methylosinus trichosporium. Organism_taxid: 426. Organism_taxid: 426
Chains: B, D, G (383 residues) CATH domain: 1.10.620.20
1mhz
Methane monooxygenase hydroxylase
Source: Methylosinus trichosporium. Organism_taxid: 426. Organism_taxid: 426
Chains: B, D, G (383 residues) CATH domain: 1.10.620.20
1mmo
Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: B, C, D, E, G, H (384 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group ACY is 50.00% similar to enzyme product Methanol
1mty
Methane monooxygenase hydroxylase from methylococcus capsulatus (bath)
Source: Methylococcus capsulatus str. Bath. Organism_taxid: 243233. Strain: bath. Cellular_location: cytoplasm. Cellular_location: cytoplasm
Chains: D, E, B, C, G, H (512 residues) CATH domain: 1.10.620.20
1tvc
Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath)
Source: Methylococcus capsulatus. Organism_taxid: 414. Gene: mmoc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (250 residues) CATH domains: 2.40.30.10 3.40.50.80
Bound ligand:   Het Group FDA is 71.00% similar to enzyme reactant NADPH
1xmf
Structure of mn(ii)-soaked apo methane monooxygenase hydroxy crystals from m. Capsulatus (bath)
Source: Methylococcus capsulatus. Organism_taxid: 243233. Strain: bath. Other_details: gene mmox. Other_details: gene mmoy. Other_details: gene mmoz
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xmg
Crystal structure of apo methane monooxygenase hydroxylase f capsulatus (bath)
Source: Methylococcus capsulatus. Organism_taxid: 243233. Strain: bath. Other_details: gene mmox. Other_details: gene mmoy. Other_details: gene mmoz
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xmh
Structure of co(ii) reconstituted methane monooxygenase hydr from m. Capsulatus (bath)
Source: Methylococcus capsulatus. Organism_taxid: 243233. Strain: bath. Other_details: gene mmox. Other_details: gene mmoy. Other_details: gene mmoz
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xu3
Soluble methane monooxygenase hydroxylase-soaked with bromop
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xu5
Soluble methane monooxygenase hydroxylase-phenol soaked
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xvb
Soluble methane monooxygenase hydroxylase: 6-bromohexanol so structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xvc
Soluble methane monooxygenase hydroxylase: 8-bromooctanol soaked structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (511 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group FMT is 66.00% similar to enzyme product Methanol
1xvd
Soluble methane monooxygenase hydroxylase: 4-fluorophenol so structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xve
Soluble methane monooxygenase hydroxylase: 3-bromo-3- butenol soaked structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
1xvf
Soluble methane monooxygenase hydroxylase: chloropropanol soaked structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group 3CL is 40.00% similar to enzyme product Methanol
1xvg
Soluble methane monooxygenase hydroxylase: bromoethanol soak structure
Source: Methylococcus capsulatus. Organism_taxid: 414. Organism_taxid: 414
Chains: A, B, C, D, E, F (510 residues) CATH domain: 1.10.620.20
Bound ligand:   Het Group BRJ is 50.00% similar to enzyme product Methanol
3rgb
Crystal structure of particulate methane monooxygenase from methylococcus capsulatus (bath)
Source: Methylococcus capsulatus. Organism_taxid: 414. Strain: bath. Strain: bath
Chains: C, K, G (213 residues) CATH domain: 1.20.1050.50
4gam
Complex structure of methane monooxygenase hydroxylase and r subunit
Source: Methylococcus capsulatus. Organism_taxid: 243233. Strain: methylococcus capsulatus. Other_details: mmoh : purified from the original bacteria. Strain: atcc 33009 / ncimb 11132 / bath. Gene: mmob, mca1196. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: B, A, C, G, F, H, L, K, M, Q, P, R (388 residues) CATH domain: 1.10.620.20