_EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase. 35 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,663 PDB entries]
EC 1.14.-.- Acting on paired donors, with incorporation or reduction of molecu [1,436 PDB entries]
EC 1.14.13.- With Nadh or nadph as one donor, and incorporation of one atom of [661 PDB entries]
EC 1.14.13.2 4-hydroxybenzoate 3-monooxygenase. [35 PDB entries]    
1bf3

Pathway: Benzoate Metabolism
Reaction: 4-hydroxybenzoate + Nadph + O(2) = protocatechuate + NADP(+) + H(2)O.
 


4-hydroxybenzoate
+
NADPH
+
O(2)
=
protocatechuate
+
NADP(+)
+ H(2)O
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): p-hydroxybenzoate hydroxylase. p-hydroxybenzoic acid hydroxylase. Para-hydroxybenzoate hydroxylase.
Cofactor(s): Fad.
 

FAD
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.
Comments: Most enzymes from Pseudomonas are highly specific for NAD(P)H (cf. Ec 1.14.13.33).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 35 PDB entries in enzyme class E.C.1.14.13.2

  PDB code Protein
1bf3
P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 rep ser (c116s) and arg 42 replaced by lys (r42k), in complex w and 4-hydroxybenzoic acid
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1bgj
P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 rep ser (c116s) and his 162 replaced by arg (h162r), in complex and 4-hydroxybenzoic acid
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1bgn
P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 rep ser (c116s) and arg 269 replaced by thr (r269t), in complex and 4-hydroxybenzoic acid
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1bkw
P-hydroxybenzoate hydroxylase (phbh) mutant with cys116 repl ser (c116s) and arg44 replaced by lys (r44k), in complex wi 4-hydroxybenzoic acid
Source: Pseudomonas fluorescens. Organism_taxid: 294. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1cc4
Phe161 and arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability
Source: Pseudomonas fluorescens. Organism_taxid: 294. Strain: tg2. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1cc6
Phe161 and arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability
Source: Pseudomonas fluorescens. Organism_taxid: 294. Strain: tg2. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1cj2
Mutant gln34arg of para-hydroxybenzoate hydroxylase
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1cj3
Mutant tyr38glu of para-hydroxybenzoate hydroxylase
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (392 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1cj4
Mutant q34t of para-hydroxybenzoate hydroxylase
Source: Pseudomonas fluorescens. Organism_taxid: 294. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (392 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1d7l
Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase sub with a series of 8-substituted flavins
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group RFL is 92.86% similar to enzyme cofactor FAD
1dob
The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1doc
The mobil flavin of 4-oh benzoate hydroxylase: motion of a p group regulates catalysis
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1dod
The mobil flavin of 4-oh benzoate hydroxylase: motion of a p group regulates catalysis
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1doe
The mobil flavin of 4-oh benzoate hydroxylase: motion of a p group regulates catalysis
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1ius
P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1iut
P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1iuu
P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1iuv
P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenz 5.0
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1iuw
P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenz 7.4
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1iux
P-hydroxybenzoate hydroxylase complexed with 4-4-hydroxybenz 9.4
Source: Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1k0i
Pseudomonas aeruginosa phbh r220q in complex with 100mm phb
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1k0j
Pseudomonas aeruginosa phbh r220q in complex with NADPH and ohb
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group NDP corresponds to enzyme reactant NADPH
  Het Group FAD corresponds to enzyme cofactor FAD
1k0l
Pseudomonas aeruginosa phbh r220q free of p-ohb
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligand:   Het Group FAD corresponds to enzyme cofactor FAD
1pbb
Crystal structures of wild-type p-hydroxybenzoate hydroxylas complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2 4-aminobenzoate and of the try222ala mutant, complexed with hydroxy-4-aminobenzoate. Evidence for a proton channel and binding mode of the flavin ring
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group DOB is 90.91% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pbc
Crystal structures of wild-type p-hydroxybenzoate hydroxylas complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2 4-aminobenzoate and of the try222ala mutant, complexed with hydroxy-4-aminobenzoate. Evidence for a proton channel and binding mode of the flavin ring
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group BHA is 75.00% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pbd
Crystal structures of wild-type p-hydroxybenzoate hydroxylas complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2 4-aminobenzoate and of the try222ala mutant, complexed with hydroxy-4-aminobenzoate. Evidence for a proton channel and binding mode of the flavin ring
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PAB is 81.82% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pbe
Crystal structure of the p-hydroxybenzoate hydroxylase-subst complex refined at 1.9 angstroms resolution. Analysis of th substrate and enzyme-product complexes
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pbf
Crystal structures of wild-type p-hydroxybenzoate hydroxylas complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2 4-aminobenzoate and of the try222ala mutant, complexed with hydroxy-4-aminobenzoate. Evidence for a proton channel and binding mode of the flavin ring
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group BHA is 75.00% similar to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pdh
Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAS corresponds to enzyme cofactor FAD
1phh
Crystal structure of p-hydroxybenzoate hydroxylase complexed reaction product 3,4-dihydroxybenzoate
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group DHB corresponds to enzyme product 3,4-dihydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pxa
Crystal structures of mutant pseudomonas aeruginosa p-hydrox hydroxylase: the tyr201phe, tyr385phe, and asn300asp varian
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pxb
Crystal structures of mutant pseudomonas aeruginosa p-hydrox hydroxylase: the tyr201phe, tyr385phe, and asn300asp varian
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1pxc
Crystal structures of mutant pseudomonas aeruginosa p-hydrox hydroxylase: the tyr201phe, tyr385phe, and asn300asp varian
Source: Pseudomonas aeruginosa. Organism_taxid: 287
Chain: A (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
1ykj
A45g p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bo
Source: Pseudomonas aeruginosa. Organism_taxid: 287. Gene: poba. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (394 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group FAD corresponds to enzyme cofactor FAD
2phh
The coenzyme analogue adenosine 5-diphosphoribose displaces active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation
Source: Pseudomonas fluorescens. Organism_taxid: 294
Chain: A (391 residues) CATH domains: 3.50.50.60 3.30.9.10
Bound ligands:   Het Group PHB corresponds to enzyme reactant 4-hydroxybenzoate
  Het Group APR is 71.43% similar to enzyme reactant NADPH