_EC 1.13.12.7 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing). 15 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,621 PDB entries]
EC 1.13.-.- Acting on single donors with incorporation of molecular oxygen. [303 PDB entries]
EC 1.13.12.- With incorporation of one atom of oxygen. [32 PDB entries]
EC 1.13.12.7 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing). [15 PDB entries]    
1ba3

Pathway: Photinus-luciferin 4-monooxygenase (ATP-hydrolysing)
Reaction: Photinus luciferin + O(2) + Atp = oxidized Photinus luciferin + CO(2) + Amp + diphosphate + light.
 


Photinus luciferin
+
O(2)
+
ATP
=
oxidized Photinus luciferin
+
CO(2)
+
AMP
+
diphosphate
+ light
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Firefly luciferase. Luciferase. Photinus pyralis luciferase.
Comments: Photinus (firefly) is a bioluminescent insect. The first step in the reaction is the formation of an acid anhydride between the carboxylic group and Amp, with the release of diphosphate. The enzyme may be assayed by measurement of light emission.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 15 PDB entries in enzyme class E.C.1.13.12.7

  PDB code Protein
1ba3
Firefly luciferase in complex with bromoform
Source: Photinus pyralis. Common eastern firefly. Organism_taxid: 7054. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (540 residues) CATH domains: 3.40.50.980 3.40.50.980 2.30.38.10 3.30.300.30 4.10.8.10
1lci
Firefly luciferase
Source: Photinus pyralis. Common eastern firefly. Organism_taxid: 7054. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (523 residues) CATH domains: 3.40.50.980 3.40.50.980 2.30.38.10 3.30.300.30
2d1q
Crystal structure of the thermostable japanese firefly luciferase complexed with mgatp
Source: Luciola cruciata. Japanese firefly. Organism_taxid: 7051. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (531 residues) CATH domains: 2.30.38.10 3.40.50.980 3.40.50.980
Bound ligand:   Het Group AMP corresponds to enzyme product AMP
2d1r
Crystal structure of the thermostable japanese firefly luciferase complexed with oxyluciferin and amp
Source: Luciola cruciata. Japanese firefly. Organism_taxid: 7051. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (539 residues) CATH domains: 2.30.38.10 3.40.50.980 3.40.50.980
Bound ligands:   Het Group OLU corresponds to enzyme product oxidized Photinus luciferin
  Het Group AMP corresponds to enzyme product AMP
2d1s
Crystal structure of the thermostable japanese firefly luciferase complexed with high-energy intermediate analogue
Source: Luciola cruciata. Japanese firefly. Organism_taxid: 7051. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (539 residues) CATH domains: 2.30.38.10 3.40.50.980 3.40.50.980
Bound ligand:   Het Group SLU is 43.00% similar to enzyme product AMP
2d1t
Crystal structure of the thermostable japanese firefly luciferase red-color emission s286n mutant complexed with high-energy intermediate analogue
Source: Luciola cruciata. Japanese firefly. Organism_taxid: 7051. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (539 residues) CATH domains: 2.30.38.10 3.40.50.980 3.40.50.980
Bound ligand:   Het Group SLU is 43.00% similar to enzyme product AMP
3iep
Firefly luciferase apo structure (p41 form)
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054
Chain: A (428 residues) CATH domain: Unassigned
3ier
Firefly luciferase apo structure (p41 form) with peg 400 bound
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054
Chain: A (427 residues) CATH domain: Unassigned
3ies
Firefly luciferase inhibitor complex
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054
Chain: A (431 residues) CATH domain: Unassigned
Bound ligand:   Het Group M24 is 53.00% similar to enzyme product AMP
3qya
Crystal structure of a red-emitter mutant of lampyris turkes luciferase
Source: Lampyris turkestanicus. Iranian firefly. Organism_taxid: 293978. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (427 residues) CATH domain: Unassigned
Bound ligand:   Het Group EDO is 40.00% similar to enzyme product CO(2)
3rix
1.7a resolution structure of a firefly luciferase-aspulvinon inhibitor complex
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054
Chain: A (428 residues) CATH domain: Unassigned
4e5d
2.2a resolution structure of a firefly luciferase-benzothiaz inhibitor complex
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054. Other_details: lyophilized power from sigma-aldrich catalog
Chain: A (425 residues) CATH domain: Unassigned
Bound ligand:   Het Group 0NJ is 47.83% similar to enzyme product oxidized
4g36
Photinus pyralis luciferase in the adenylate-forming conform bound to dlsa
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (522 residues) CATH domains: 2.30.38.10 3.40.50.980 3.40.50.980
Bound ligand:   Het Group SLU is 43.18% similar to enzyme product AMP
4g37
Structure of cross-linked firefly luciferase in second catal conformation
Source: Photinus pyralis. North american firefly. Organism_taxid: 7054. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (533 residues) CATH domains: Unassigned Unassigned
Bound ligands:   Het Group XLX is 52.94% similar to enzyme product oxidized
  Het Group SLU is 43.18% similar to enzyme product AMP
4m46
Crystal structure of a green-emitter native of lampyris turk luciferase
Source: Lampyris turkestanicus. Iranian firefly. Organism_taxid: 293978. Strain: lampyris. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chain: A (427 residues) CATH domain: Unassigned