_EC 1.13.11.3 Protocatechuate 3,4-dioxygenase. 47 PDB entries  
EC 1.-.-.- Oxidoreductases. [8,840 PDB entries]
EC 1.13.-.- Acting on single donors with incorporation of molecular oxygen. [310 PDB entries]
EC 1.13.11.- With incorporation of two atoms of oxygen. [275 PDB entries]
EC 1.13.11.3 Protocatechuate 3,4-dioxygenase. [47 PDB entries]    
1eo2

Pathway: Benzoate Metabolism
Reaction: 3,4-dihydroxybenzoate + O(2) = 3-carboxy-cis,cis-muconate.
 


3,4-dihydroxybenzoate
+
O(2)
=
3-carboxy-cis,cis-muconate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Protocatechuate oxygenase.
Cofactor(s): Fe cation.
Comments: The enzyme, which participates in the degradation of aromatic compounds, catalyzes the intradiol addition of both oxygen atoms from molecular oxygen, resulting in ortho-cleavage of the aromatic ring. The type of cleavage leads to mineralization via the intermediate 3-oxoadipate. Formerly Ec 1.13.1.3 and Ec 1.99.2.3.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 47 PDB entries in enzyme class E.C.1.13.11.3

  PDB code Protein
1eo2
Crystal structure of acinetobacter sp. Adp1 protocatechuate dioxygenase
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: cloned and expressed in e.Coli. Expression_system_taxid: 562
Chains: A, B (202 residues) CATH domain: 2.60.130.10
1eo9
Crystal structure of acinetobacter sp. Adp1 protocatechuate dioxygenase at ph < 7.0
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, B (202 residues) CATH domain: 2.60.130.10
1eoa
Crystal structure of acinetobacter sp. Adp1 protocatechuate dioxygenase in complex with cyanide
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, B (202 residues) CATH domain: 2.60.130.10
1eob
Crystal structure of acinetobacter sp. Adp1 protocatechuate dioxygenase in complex with 3,4-dihydroxybenzoate
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: cloned and overexpressed in e.Coli. Expression_system_taxid: 562
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
1eoc
Crystal structure of acinetobacter sp. Adp1 protocatechuate dioxygenase in complex with 4-nitrocatechol
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 4NC is 57.14% similar to enzyme reactant 3,4-dihydroxybenzoate
1ykk
Protocatechuate 3,4-dioxygenase y408c mutant
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
1ykl
Protocatechuate 3,4-dioxygenase y408c mutant bound to dhb
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
1ykm
Protocatechuate 3,4-dioxygenase y408e mutant
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
1ykn
Protocatechuate 3,4-dioxygenase y408e mutant bound to dhb
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
1yko
Protocatechuate 3,4-dioxygenase y408h mutant
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
1ykp
Protocatechuate 3,4-dioxygenase y408h mutant bound to dhb
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah. Expression_system_taxid: 562
Chains: A, B, C, D, E, F, G, H, I, J, K, L (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
2bum
Crystal structure of wild-type protocatechuate 3,4- dioxygenase from acinetobacter sp. Adp1
Source: Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
2buq
Crystal structure of wild-type protocatechuate 3,4- dioxygenase from acinetobacter sp. Adp1 in complex with catechol
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group CAQ is 72.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2bur
Crystal structure of wild-type protocatechuate 3,4- dioxygenase from acinetobacter sp. Adp1 in complex with 4- hydroxybenzoate
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group PHB is 90.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2but
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r457s - apo
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
2buu
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r457s in complex with 4- nitrocatechol
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 4NC is 57.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2buv
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r457s in complex with protocatechuate
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
2buw
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r457s in complex with 4- hydroxybenzoate
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group PHB is 90.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2bux
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r133h
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
2buy
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r133h in complex with catechol
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group CAQ is 72.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2buz
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r133h in complex with 4- nitrocatechol
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 4NC is 57.00% similar to enzyme reactant 3,4-dihydroxybenzoate
2bv0
Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r133h in complex with protocatechuate.
Source: Acinetobacter calcoaceticus. Organism_taxid: 62977. Strain: adp1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (202 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
2pcd
Structure of protocatechuate 3,4-dioxygenase from pseudomona aeruginosa at 2.15 angstroms resolution
Source: Pseudomonas putida. Organism_taxid: 303. Organism_taxid: 303
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
3lkt
Tyrosine 447 of protocatechuate 3,4-dioxygenase controls eff progress through catalysis
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group GOL is 41.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3lmx
Tyrosine 447 of protocatechuate 34,-dioxygenase controls eff progress through catalysis
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
3lxv
Tyrosine 447 of protocatechuate 3,4-dioxygenase controls eff progress through catalysis
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 4NC is 57.14% similar to enzyme reactant 3,4-dihydroxybenzoate
3mfl
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHY is 91.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3mi1
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group GOL is 41.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3mi5
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Strain: p. Putida. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group CAQ is 72.73% similar to enzyme reactant 3,4-dihydroxybenzoate
3mv4
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag, pcah. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, B, C, M, N, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group GOL is 41.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3mv6
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag, pcah. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, B, C, M, N, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
3pca
Structure of protocatechuate 3,4-dioxygenase complexed with dihydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHB corresponds to enzyme reactant 3,4-dihydroxybenzoate
3pcb
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 3HB is 90.91% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcc
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group PHB is 90.91% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcd
Protocatechuate 3,4-dioxygenase y447h mutant
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Gene: pcah. Expressed in: pseudomonas fluorescens. Expression_system_taxid: 294.
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
3pce
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxyphenylacetate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 3HP is 83.33% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcf
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group FHB is 83.33% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcg
Structure of protocatechuate 3,4-dioxygenase complexed with inhibitor 4-hydroxyphenylacetate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group 4HP is 83.33% similar to enzyme reactant 3,4-dihydroxybenzoate
3pch
Structure of protocatechuate 3,4-dioxygenase complexed with 4-hydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Atcc: 23975
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group CHB is 83.33% similar to enzyme reactant 3,4-dihydroxybenzoate
3pci
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxybenzoate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group IHB is 83.33% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcj
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxyisonicotinic acid n-oxide
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group INO is 69.23% similar to enzyme reactant 3,4-dihydroxybenzoate
3pck
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxynicotinic acid n-oxide
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group NNO is 69.23% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcl
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxyisonicotinic acid n-oxide and cyanide
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group INO is 69.23% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcm
Structure of protocatechuate 3,4-dioxygenase complexed with hydroxynicotinic acid n-oxide and cyanide
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group NNO is 69.23% similar to enzyme reactant 3,4-dihydroxybenzoate
3pcn
Structure of protocatechuate 3,4-dioxygenase complexed with dihydroxyphenylacetate
Source: Pseudomonas putida. Organism_taxid: 303. Atcc: 23975. Other_details: previously classified as pseudomonas aerugin
Chains: A, M, B, N, C, O, D, P, E, Q, F, R (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group DHY is 91.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3t63
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: pcah.
Chains: A, B, C, M, N, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group GOL is 41.67% similar to enzyme reactant 3,4-dihydroxybenzoate
3t67
Axial ligand swapping in double mutant maintains intradiol-c chemistry in protocatechuate 3,4-dioxygenase
Source: Pseudomonas putida. Organism_taxid: 303. Gene: pcag. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcah.
Chains: A, B, C, M, N, O (200 residues) CATH domain: 2.60.130.10
Bound ligand:   Het Group CAQ is 72.73% similar to enzyme reactant 3,4-dihydroxybenzoate