_EC 1.1.1.35 3-hydroxyacyl-CoA dehydrogenase. 27 PDB entries  
EC 1.-.-.- Oxidoreductases. [7,297 PDB entries]
EC 1.1.-.- Acting on the CH-OH group of donors. [1,523 PDB entries]
EC 1.1.1.- With NAD(+) or NADP(+) as acceptor. [1,373 PDB entries]
EC 1.1.1.35 3-hydroxyacyl-CoA dehydrogenase. [27 PDB entries]    
1e3s

Reaction: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + Nadh.
 


(S)-3-hydroxyacyl-CoA
+
NAD(+)
=
3-oxoacyl-CoA
+
NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Other name(s): Beta-hydroxyacyl dehydrogenase. Beta-keto-reductase.
Comments: Also oxidizes S-3-hydroxyacyl-N-acylthioethanolamine and S-3- hydroxyacylhydrolipoate. Some enzymes act, more slowly, with NADP(+). Broad specificity to acyl chain-length (cf. Ec 1.1.1.211).
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 27 PDB entries in enzyme class E.C.1.1.1.35

  PDB code Protein
1e3s
Rat brain 3-hydroxyacyl-coa dehydrogenase binary complex with nadh
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: brain. Organelle: mitochonria. Cellular_location: cytoplasm. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B, C, D (241 residues) CATH domain: 3.40.50.720
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
1e3w
Rat brain 3-hydroxyacyl-coa dehydrogenase binary complex with nadh and 3-keto butyrate
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: brain. Organelle: mitochonria. Cellular_location: cytoplasm. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Chains: A, B, C, D (251 residues) CATH domain: 3.40.50.720
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
1f0y
L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: heart. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligands:   Het Group CAA is 96.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group NAD corresponds to enzyme reactant NAD(+)
1f12
L-3-hydroxyacyl-coa dehydrogenase complexed with 3- hydroxybutyryl-coa
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: heart. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: protein was expressed with a c-terminal hexameric histidine tag.
Chains: A, B (293 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group 3HC is 96.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
1f14
L-3-hydroxyacyl-coa dehydrogenase (apo)
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: heart. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: protein was expressed with a c-terminal hexameric histidine tag.
Chains: A, B (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
1f17
L-3-hydroxyacyl-coa dehydrogenase complexed with nadh
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: heart. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: protein was expressed with a c-terminal hexameric histidine tag.
Chains: A, B (293 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group NAI corresponds to enzyme reactant NAD(+)
1f67
Molecular modeling of human type 10 17beta-hydroxysteroid dehydrogenase
Source: Homo sapiens. Human
Chain: A (261 residues)
Bound ligand:   Het Group NAH corresponds to enzyme product NADH
1il0
X-ray crystal structure of the e170q mutant of human l-3- hydroxyacyl-coa dehydrogenase
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hcdh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligands:   Het Group CAA is 96.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group NAD corresponds to enzyme reactant NAD(+)
1lsj
Crystal structure of the e110q mutant of l-3-hydroxyacyl- coa dehydrogenase in complex with NAD
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hcdh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
1lso
Crystal structure of the s137a mutant of l-3-hydroxyacyl- coa dehydrogenase in complex with NAD
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hcdh. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
1so8
Abeta-bound human abad structure [also known as 3-hydroxyacy dehydrogenase type ii (type ii hadh), endoplasmic reticulum associated amyloid beta-peptide binding protein (erab)]
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hadh2, erab, xh98g2, schad. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Chain: A (208 residues) CATH domain: 3.40.50.720
1u7t
Crystal structure of abad/hsd10 with a bound inhibitor
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hadh2, erab, xh98g2, schad. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B, C, D (255 residues) CATH domain: 3.40.50.720
Bound ligands:   Het Group NAD is 50.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group TDT is 62.00% similar to enzyme reactant NAD(+)
1wdl
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4)
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (715 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligands:   Het Group ACO is 74.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group NAD is 79.00% similar to enzyme reactant NAD(+)
1wdm
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3)
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (707 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligands:   Het Group ACO is 94.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group NAD corresponds to enzyme reactant NAD(+)
1zcj
Crystal structure of 3-hydroxyacyl-coa dehydrogenase
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chain: A (459 residues) CATH domains: Unassigned 3.40.50.720 Unassigned 1.10.1040.10
2d3t
Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form v
Source: Pseudomonas fragi. Organism_taxid: 296. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: A, B (708 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligands:   Het Group ACO is 94.00% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
  Het Group NAD corresponds to enzyme reactant NAD(+)
2o23
The structure of wild-type human hadh2 (17beta-hydroxysteroi dehydrogenase type 10) bound to NAD+ at 1.2 a
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hadh2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chains: A, B (248 residues) CATH domain: 3.40.50.720
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
2wtb
Arabidopsis thaliana multifuctional protein, mfp2
Source: Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (676 residues) CATH domains: 3.90.226.10 3.40.50.720
3had
Biochemical characterization and structure determination of heart short chain l-3-hydroxyacyl coa dehydrogenase provide into catalytic mechanism
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: heart. Organelle: mitochondrial. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: protein was expressed with a c-terminal hexa histidine tag.
Chains: A, B (293 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
3hdh
Pig heart short chain l-3-hydroxyacyl coa dehydrogenase revisited: sequence analysis and crystal structure determination
Source: Sus scrofa. Pig. Organism_taxid: 9823. Organ: heart. Organelle: mitochondrial
Chains: A, B, C (291 residues) CATH domains: 3.40.50.720 1.10.1040.10
Bound ligand:   Het Group NAD corresponds to enzyme reactant NAD(+)
3ppi
Crystal structure of 3-hydroxyacyl-coa dehydrogenase type-2 mycobacterium avium
Source: Mycobacterium avium. Organism_taxid: 243243. Strain: 104. Gene: mav_1812. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B, C, D (258 residues) CATH domain: 3.40.50.720
3rqs
Crystal structure of human l-3- hydroxyacyl-coa dehydrogenas (ec1.1.1.35) from mitochondria at the resolution 2.0 a, nor structural genomics consortium target hr487, mitochondrial partnership
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: hadh, had, hadhsc, schad. Expressed in: escherichia coli. Expression_system_taxid: 562
Chains: A, B (292 residues) CATH domains: 3.40.50.720 1.10.1040.10
3zw8
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) in apo form
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
3zwa
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (727 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group 3H9 is 92.98% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
3zwb
Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: peroxisome. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: A, B (725 residues) CATH domains: 3.90.226.10 3.40.50.720
Bound ligand:   Het Group TC6 is 91.23% similar to enzyme reactant (S)-3-hydroxyacyl-CoA
4j0e
Crystal structure of 3-hydroxyacyl-coa dehydrogenase from caenorhadbitis elegans in p1 space group
Source: Caenorhabditis elegans. Nematode. Organism_taxid: 6239. Gene: f54c8.1. Expressed in: escherichia coli. Expression_system_taxid: 562. Pet-22b)
Chains: A, B (296 residues) CATH domains: 3.40.50.720 1.10.1040.10
4j0f
Crystal structure of 3-hydroxyacyl-coa dehydrogenase from caenorhadbitis elegans in p212121 space group
Source: Caenorhabditis elegans. Nematode. Organism_taxid: 6239. Gene: f54c8.1. Expressed in: escherichia coli. Expression_system_taxid: 562. Pet-22b)
Chains: A, B (287 residues) CATH domains: 3.40.50.720 1.10.1040.10