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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0294 and M0142

These two reactions have a combined similarity of 0.33


M0294

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Comparison

M0142

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EC 1.3.5.1
succinate dehydrogenase (ubiquinone)
Sub-Class EC 1.18.1.2
ferredoxin-NADP+ reductase

Image of ubiquniol

Image of fumarate

right arrow

Image of ubiquinone

Image of succinate

ubiquniol
C00390
CHEBI:17976
fumarate
C00122
CHEBI:37154
ubiquinone
C00399
CHEBI:16389
succinate
C00042
CHEBI:30031
0.2

Image of NADPH

Image of adrenodoxin

right arrow

Image of proton

Image of NADP

Image of reduced adrenodoxin

NADPH
C00005
CHEBI:16474
2 adrenodoxin
C00667
CHEBI:16341
proton
C00080
CHEBI:24636
NADP
C00006
CHEBI:18009
2 reduced adrenodoxin
C00662
CHEBI:16906

Catalytic CATH Codes

3.50.50.60
0.0.0.0

Catalytic CATH Codes

3.40.50.720
3.50.50.60

Active Site



0.14112

Active Site



Catalytic Residues

Type Number Chain Location of Function
Phe 130 A Side Chain
Leu 263 A Side Chain
Glu 266 A Side Chain
Gln 251 A Side Chain
His 253 A Side Chain
His 364 A Side Chain
Arg 408 A Side Chain
Arg 297 A Side Chain
0.1428

Catalytic Residues

Type Number Chain Location of Function
His 55 A Side Chain
Asp 159 A Side Chain
Ile 376 A Main Chain Amide
Main Chain Carbonyl
Thr 377 A Main Chain Amide
Main Chain Carbonyl

Organic Cofactors

Type Identity Chain
FAD FAD 1001 A

Organic Cofactors

Type Identity Chain
FAD FAD 461 A

Metal Cofactors

Type Het group Number Chain
iron SF4 1003 B
iron F3S 1004 B
iron No Available PDB Information

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 3 steps

0.4285

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0294.stg01

Ubiquinol acts as a two electron donor to the FAD cofactor, forming anionic FADH.
0.47 Step 1
GIF of Reaction Step M0142.stg01

A hydride ion is transferred from the substrate NADP to the cofactor, FAD461.
Step 2
GIF of Reaction Step M0294.stg02

The FADH N5 acts as a hydride donor to the polarised double bond in fumarate. A proton is simultaneously abstracted from Arg297 to saturate the double bond, forming succinate.
0.16 Step 2
GIF of Reaction Step M0142.stg02

A single electron is transferred from the FAD461 to one of the substrate adrenodoxin dmers via the main chains of Ile376 and Thr377 and a second adrenodoxin dimer.
Step 3
GIF of Reaction Step M0294.stg03

The catalytic acid/base is reprotonated by a solvent molecule.
0 Step 3
GIF of Reaction Step M0142.stg03

FAD461 is deprotonated by a water molecule activated by Asp159. This results in the second electron transfer from FAD461 to the second adrenodoxin dimer via the main chains of Ile376 and Thr377.

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