spacer

The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0282 and M0327

These two reactions have a combined similarity of 0.05


M0282

View
Comparison

M0327

View
EC 2.7.12.2
mitogen-activated protein kinase kinase
Sub-SubClass EC 2.7.3.-
protein histidine kinase (CheA)

Image of serine protein

Image of ATP

right arrow

Image of phosphorylated serine protein

Image of ADP

serine protein
C02189
CHEBI:29999
ATP
C00002
CHEBI:30616
phosphorylated serine protein
X00134
CHEBI:61975
ADP
C00008
CHEBI:456216
0.5

Image of ATP

Image of Asp-CheY

right arrow

Image of pAsp-CheY

Image of ADP

ATP
C00002
CHEBI:57299
Asp-CheY
X00148
pAsp-CheY
X00149
ADP
C00008
CHEBI:456216

Catalytic CATH Codes

1.10.510.10

Catalytic CATH Codes

3.30.200.20
0.0.0.0

Active Site



0

Active Site



Catalytic Residues

Type Number Chain Location of Function
Asp 194 A Side Chain
Asp 221 A Side Chain
0

Catalytic Residues

Type Number Chain Location of Function
Asn 409 A Side Chain
His 45 A Side Chain
Glu 67 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
magnesium MG 536 A

Metal Cofactors

Type Het group Number Chain
magnesium MG 382 A

Reaction occurs across 3 steps

0

Reaction occurs across 2 steps

Step 1
GIF of Reaction Step M0282.stg01

The serine residue of the protein substrate attacks the terminal, pentavalent phosphorous of ATP.
0 Step 1
GIF of Reaction Step M0327.stg01

His45, activated by Glu67, acts as a nucleophile towards the terminal phosphate group of ATP.
Step 2
GIF of Reaction Step M0282.stg02

Asp194 abstracts a proton from the phospho-serine residue.
0 Step 2
GIF of Reaction Step M0327.stg02

The nucleophilic Asp of chemoreceptor Y (CheY) attacks the phosphorylated His45, removing the functional group and regenerating the active site.
Step 3
GIF of Reaction Step M0282.stg03

Asp194 relays the proton to ADP, regenerating the active site.
N/A Step 3
No Step with this number present

spacer
spacer