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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0282 and M0246

These two reactions have a combined similarity of 0.48


M0282

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Comparison

M0246

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EC 2.7.12.2
mitogen-activated protein kinase kinase
Sub-SubClass EC 2.7.10.1
receptor protein-tyrosine kinase

Image of serine protein

Image of ATP

right arrow

Image of phosphorylated serine protein

Image of ADP

serine protein
C02189
CHEBI:29999
ATP
C00002
CHEBI:30616
phosphorylated serine protein
X00134
CHEBI:61975
ADP
C00008
CHEBI:456216
1

Image of ATP

Image of protein tyrosine

right arrow

Image of protein tyrosine phosphate

Image of ADP

ATP
C00002
CHEBI:30616
protein tyrosine
C00585
CHEBI:32789
protein tyrosine phosphate
C01167
ADP
C00008
CHEBI:456216

Catalytic CATH Codes

1.10.510.10

Catalytic CATH Codes

1.10.510.10

Active Site



0.4849

Active Site



Catalytic Residues

Type Number Chain Location of Function
Asp 194 A Side Chain
Asp 221 A Side Chain
0.5

Catalytic Residues

Type Number Chain Location of Function
Asp 1132 A Side Chain
Arg 1136 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
magnesium MG 536 A

Metal Cofactors

Type Het group Number Chain
magnesium MG 301 _
magnesium MG 302 _

Reaction occurs across 3 steps

0.4

Reaction occurs across 2 steps

Step 1
GIF of Reaction Step M0282.stg01

The serine residue of the protein substrate attacks the terminal, pentavalent phosphorous of ATP.
0 Step 1
GIF of Reaction Step M0246.stg01

The gamma phosphate of the ATP substrate eliminates itself in a heterolysis, resulting in the transfer of a proton from the gamma phosphate to the beta phosphate and formation of ADP and the highly reactive metaphosphate anion.
Step 2
GIF of Reaction Step M0282.stg02

Asp194 abstracts a proton from the phospho-serine residue.
0.66 Step 2
GIF of Reaction Step M0246.stg02

The protein tyrosine initiated a nucleophilic attack on the metaphosphate intermediate in an addition reaction, the resulting species then deprotonates the tyrosine phenol group to form the final product.
Step 3
GIF of Reaction Step M0282.stg03

Asp194 relays the proton to ADP, regenerating the active site.
N/A Step 3
No Step with this number present

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