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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0251 and M0295

These two reactions have a combined similarity of 0.13


M0251

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Comparison

M0295

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EC 2.1.1.63
methylated-DNA--[protein]-cysteine S-methyltransferase
EC 2.1.1.63
methylated-DNA--[protein]-cysteine S-methyltransferase

Image of DNA containing 6-O-methylguanine

Image of Protein L-cysteine

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Image of DNA containing guanine

Image of Protein S-methyl-L-cysteine

DNA containing 6-O-methylguanine
C04250
Protein L-cysteine
C02743
CHEBI:32460
DNA containing guanine
C11475
CHEBI:61292
Protein S-methyl-L-cysteine
C03800
CHEBI:32648
0.2

Image of S-methyl phosphotriester

Image of protein L-cysteine

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Image of protein S-methyl-L-cysteine

Image of DNA

S-methyl phosphotriester
X00136
protein L-cysteine
C02743
CHEBI:29964
protein S-methyl-L-cysteine
C03800
CHEBI:16044
DNA
C00039
CHEBI:4705

Catalytic CATH Codes

1.10.10.10

Catalytic CATH Codes

3.40.10.10

Active Site



0.1836

Active Site



Catalytic Residues

Type Number Chain Location of Function
Glu 172 A Side Chain
His 146 A Side Chain
Cys 145 A Side Chain
Tyr 114 A Side Chain
Asn 137 A Side Chain
0.2

Catalytic Residues

Type Number Chain Location of Function
Cys 38 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

No Associated Metal Cofactors

Metal Cofactors

Type Het group Number Chain
zinc ZN 93 A

Reaction occurs across 1 steps

0.1111

Reaction occurs across 1 steps

Step 1
GIF of Reaction Step M0251.stg01

His146 deprotonates water, which in turn deprotonates Cys145. Cys145 then initiates a nucleophilic attack upon the methylated DNA in a substitution reaction. The DNA base then undergoes double bond rearrangement, resulting in the deprotonation of Tyr114.
0.11 Step 1
GIF of Reaction Step M0295.stg01

The thiolate anion undergoes nucleophilic substitution at the methyl group, irreversibly methylating the Cys38 residue.

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