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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0235 and M0309

These two reactions have a combined similarity of 0.67


M0235

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Comparison

M0309

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EC 6.1.1.19
arginine-tRNA ligase
Serial Number EC 6.1.1.5
isoleucine-tRNA ligase

Image of L-Arginine

Image of ATP

Image of tRNA(Arg)

right arrow

Image of L-Arginyl-tRNA(Arg)

Image of AMP

Image of diphosphate

L-Arginine
C00062
CHEBI:32682
ATP
C00002
CHEBI:30616
tRNA(Arg)
C01636
CHEBI:29171
L-Arginyl-tRNA(Arg)
C02163
CHEBI:18366
AMP
C00020
CHEBI:456215
diphosphate
C00013
CHEBI:18361
1

Image of L-Isoleucine

Image of ATP

Image of tRNA (Isoleucine)

right arrow

Image of Isoleucyl-tRNA

Image of AMP

Image of diphosphate

L-Isoleucine
C00407
CHEBI:17191
ATP
C00002
CHEBI:15422
tRNA (Isoleucine)
C01644
CHEBI:29174
Isoleucyl-tRNA
C03127
CHEBI:29160
AMP
C00020
CHEBI:16027
diphosphate
C00013
CHEBI:45212

Catalytic CATH Codes

3.40.50.620

Catalytic CATH Codes

3.40.50.620

Active Site



0.12852

Active Site



Catalytic Residues

Type Number Chain Location of Function
His 159 A Side Chain
Lys 156 A Side Chain
His 162 A Side Chain
0.1428

Catalytic Residues

Type Number Chain Location of Function
Lys 591 A Side Chain
Lys 594 A Side Chain
Pro 46 A Side Chain
Trp 518 A Side Chain
Trp 558 A Side Chain
Asp 85 A Side Chain
Gln 554 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
magnesium No Available PDB Information

Metal Cofactors

Type Het group Number Chain
zinc ZN 1101 A
zinc ZN 1102 A
magnesium No Available PDB Information

Reaction occurs across 3 steps

0.8333

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0235.stg01

The carboxylate oxygen of arginine is the nucleophile for in-line displacement at the alpha-phosphate of ATP. This produces pyrophosphate and an arginyl-adenylate intermediate.
1 Step 1
GIF of Reaction Step M0309.stg01

The zwitterionic leucine substrate attacks the alpha phosphate of ATP, forming an activated, adenylated, amino acid.
Step 2
GIF of Reaction Step M0235.stg02

The 2'-hydroxyl of the 3'-terminal adenosine of the tRNA molecule is the nucleophile for attack on the carbonyl carbon of the arginyl-adenylate intermediate. This forms a tetrahedral intermediate.
1 Step 2
GIF of Reaction Step M0309.stg02

The 2' end of the specific tRNA molecule attacks at the activated phospho-ester bond.
Step 3
GIF of Reaction Step M0235.stg03

The tetrahedral intermediate collapses and eliminates AMP to form L-arginyl-tRNA.
0.5 Step 3
GIF of Reaction Step M0309.stg03

The anionic, tetrahedral intermediate collapses, generating the tRNA-isoleucine adduct and AMP.

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