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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0197 and M0316

These two reactions have a combined similarity of 0.31


M0197

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Comparison

M0316

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EC 6.1.1.1
tyrosine-tRNA ligase
Sub-Class EC 6.3.4.5
argininosuccinate synthetase

Image of L-tyrosine

Image of ATP

Image of tRNA(Tyr)

right arrow

Image of L-tyrosyl-tRNA(Tyr)

Image of AMP

Image of diphosphate

L-tyrosine
C00082
CHEBI:58315
ATP
C00002
CHEBI:30616
tRNA(Tyr)
C00787
CHEBI:29182
L-tyrosyl-tRNA(Tyr)
C02839
CHEBI:29161
AMP
C00020
CHEBI:456215
diphosphate
C00013
CHEBI:33019
0.22

Image of L-citrulline

Image of ATP

Image of L-aspartate

right arrow

Image of diphosphate

Image of AMP

Image of N-(L-arginino)succinate

L-citrulline
C00327
CHEBI:58148
ATP
C00002
CHEBI:30616
L-aspartate
C00049
CHEBI:29991
diphosphate
C00013
CHEBI:18361
AMP
C00020
CHEBI:16027
N-(L-arginino)succinate
C03406
CHEBI:57472

Catalytic CATH Codes

3.40.50.620
1.10.240.10

Catalytic CATH Codes

3.40.50.620
3.90.1260.10

Active Site



0.04284

Active Site



Catalytic Residues

Type Number Chain Location of Function
Thr 40 A Side Chain
His 45 A Side Chain
His 48 A Side Chain
Lys 82 A Side Chain
Arg 86 A Side Chain
Gln 173 A Side Chain
Lys 230 A Side Chain
Lys 233 A Side Chain
Thr 234 A Side Chain
Asp 194 A Side Chain
0.0476

Catalytic Residues

Type Number Chain Location of Function
Ser 173 A Side Chain
Arg 92 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
magnesium MG(not in PDB) 1 x

Metal Cofactors

Type Het group Number Chain
magnesium No Available PDB Information

Reaction occurs across 2 steps

0.4285

Reaction occurs across 4 steps

Step 1
GIF of Reaction Step M0197.stg01

The carboxylate group of the substrate L-tyrosine acts as a nucleophile and attacks the alpha-phosphate of the ATP in a substitution reaction that liberates pyrophosphate. Mg(II) and all catalytic residues stabilise the intermediates formed. Thr234 also affects the steric outcome of the reaction.
1 Step 1
GIF of Reaction Step M0316.stg01

Citrulline attacks at the alpha phosphate of ATP, which is held in a reactive S conformation by surrounding residues.
Step 2
GIF of Reaction Step M0197.stg02

The phosphate of the tyrosine-AMP complex deprotonates the OH of the ribose ring, which acts as a nucleophile to attack the carbonyl group of the tyrosine in the tyrosine-AMP complex in a substitution reaction, liberating AMP. Mg(II), Thr40, His48, Lys82, Arg86 and Gln173 all stabilise the intermediates formed.
0.16 Step 2
GIF of Reaction Step M0316.stg02

The amine group of aspartate is deprotonated by Asp121, activating the nitrogen towards nucleophilic attack at the AMP-imine adduct. This results in the formation of a tetrahedral intermediate.
Step 3
No Step with this number present
N/A Step 3
GIF of Reaction Step M0316.stg03

Asp121A is deprotonated by the unprotonated amine group of the tetrahedral intermediate.
Step 4
No Step with this number present
N/A Step 4
GIF of Reaction Step M0316.stg04

The tetrahedral intermediate collapses, generating protonated AMP and arginosuccinate - a biosynthetic precursor of arginine.

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