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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0188 and M0116

These two reactions have a combined similarity of 0.35


M0188

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Comparison

M0116

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EC 5.1.3.2
UDP-glucose 4-epimerase
Class EC 1.6.1.2
NAD(P)+ transhydrogenase (AB-specific)

Image of UDP-galactose

right arrow

Image of UDP-glucose

UDP-galactose
C00052
CHEBI:58439
UDP-glucose
C00029
CHEBI:58367
0

Image of NADP

Image of NADH

right arrow

Image of NADPH

Image of NAD

NADP
C00006
CHEBI:18009
NADH
C00004
CHEBI:16908
NADPH
C00005
CHEBI:16474
NAD
C00003
CHEBI:15846

Catalytic CATH Codes

3.40.50.720

Catalytic CATH Codes

3.40.50.720
3.40.50.1770
3.40.50.1220

Active Site



0.1125

Active Site



Catalytic Residues

Type Number Chain Location of Function
Ser 124 A Side Chain
Tyr 149 A Side Chain
Lys 153 A Side Chain
0.125

Catalytic Residues

Type Number Chain Location of Function
Arg 127 A Side Chain
Asp 135 A Side Chain
Tyr 235 A Side Chain
Asp 132 C Side Chain

Organic Cofactors

Type Identity Chain
NAD NAD 340

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

No Associated Metal Cofactors

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 2 steps

0.5

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0188.stg01

Tyr149 deprotonates the 4-hydroxide of the substrate, resulting in an elimination of the hydride from the C4 position. The hydride then attacks the NAD cofactor in a nucleophilic addition reaction.
0.12 Step 1
GIF of Reaction Step M0116.stg01

Asp132C obtains a proton, causing a rearrangement of the active site.
Step 2
GIF of Reaction Step M0188.stg02

The intermediate undergoes a conformational change before the NAD initiates an elimination of the hydride back to the intermediate, which in turn deprotonates Tyr149.
0.53 Step 2
GIF of Reaction Step M0116.stg02

NAD eliminates a hydride ion, which adds to NADP.
Step 3
No Step with this number present
N/A Step 3
GIF of Reaction Step M0116.stg03

Asp132C releases its proton.

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