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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0172 and M0168

These two reactions have a combined similarity of 0.54


M0172

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Comparison

M0168

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EC 3.4.19.-
isoaspartyl dipeptidase
Sub-SubClass EC 3.4.13.19
membrane dipeptidase

Image of hydroxyde ion

Image of beta-aspartyl-dipeptide

right arrow

Image of amino acid

Image of L-aspartate

hydroxyde ion
C01328
CHEBI:16234
beta-aspartyl-dipeptide
X00075
amino acid
C00045
CHEBI:15705
L-aspartate
C00049
CHEBI:29991
0.75

Image of dipeptide

Image of water

right arrow

Image of amino acid

dipeptide
C00107
CHEBI:46761
water
C00001
CHEBI:15377
2 amino acid
C00045
CHEBI:59869

Catalytic CATH Codes

3.20.20.140

Catalytic CATH Codes

3.20.20.140

Active Site



0.45

Active Site



Catalytic Residues

Type Number Chain Location of Function
Asp 285 A Side Chain
0.5

Catalytic Residues

Type Number Chain Location of Function
His 152 A Side Chain
Asp 288 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
zinc ZN 800 x
zinc ZN 801 x

Metal Cofactors

Type Het group Number Chain
zinc ZN 401 A
zinc ZN 402 A

Reaction occurs across 2 steps

0.5555

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0172.stg01

Asp285 deprotonates the zinc activated hydroxide group. This then attacks the carbonyl carbon of the peptide bond in a nucleophilic addition.
0.25 Step 1
GIF of Reaction Step M0168.stg01

Asp288 deprotonates the zinc activated water molecule.
Step 2
GIF of Reaction Step M0172.stg02

The oxyanion initiates an elimination that cleaves the peptide bond. The amine side of the bond then deprotonates Asp285.
0.11 Step 2
GIF of Reaction Step M0168.stg02

The activated hydroxide attacks the peptide carbonyl in a nucleophilic addition.
Step 3
No Step with this number present
N/A Step 3
GIF of Reaction Step M0168.stg03

The oxyanion initiates an elimination that cleaves the peptide bond, releasing the amino acid products, the N-terminus then protonates from Asp288

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