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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0171 and M0167

These two reactions have a combined similarity of 0.60


M0171

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Comparison

M0167

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EC 3.4.17.1
carboxypeptidase A
Sub-SubClass EC 3.4.11.10
bacterial leucyl aminopeptidase

Image of protein

Image of water

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Image of proton

Image of amino acid

Image of protein

protein
C00017
CHEBI:33711
water
C00001
CHEBI:15377
proton
C00080
CHEBI:24636
amino acid
C00045
CHEBI:59814
protein
C00017
CHEBI:33711
0.83

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Image of water

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Image of amino acid

Image of protein (n-1)

protein
C00017
CHEBI:36080
water
C00001
CHEBI:15377
amino acid
C00045
CHEBI:59814
protein (n-1)
X00143

Catalytic CATH Codes

3.40.630.10

Catalytic CATH Codes

3.40.630.10

Active Site



0.4652

Active Site



Catalytic Residues

Type Number Chain Location of Function
Arg 127 A Side Chain
Glu 270 A Side Chain
0.5

Catalytic Residues

Type Number Chain Location of Function
Glu 151 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
zinc ZN 1308 A

Metal Cofactors

Type Het group Number Chain
zinc ZN 901 A
zinc ZN 902 A

Reaction occurs across 3 steps

0.625

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0171.stg01

Glu270 deprotonates the zinc activated water molecule. This activated hydroxide then attacks the carbonyl carbon of the peptide bond in a nucleophilic addition.
0.5 Step 1
GIF of Reaction Step M0167.stg01

Glu151 deprotonates the zinc activated water molecule.
Step 2
GIF of Reaction Step M0171.stg02

The oxyanion initiates an elimination that cleaves the peptide bond. The amine side of the bond then deprotonates the carboxylic side.
0 Step 2
GIF of Reaction Step M0167.stg02

The activated hydroxide attacks the peptide carbonyl in a nucleophilic addition.
Step 3
GIF of Reaction Step M0171.stg03

Water deprotonates Glu270, regenerating the active site.
0.2 Step 3
GIF of Reaction Step M0167.stg03

The oxyanion initiates an elimination that cleaves the peptide bond and releases the protein's new N-terminal amino acid, which protonates from Glu151, and the amino acid product.

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