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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0111 and M0082

These two reactions have a combined similarity of 0.67


M0111

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Comparison

M0082

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EC 1.4.7.1
glutamate synthase (ferredoxin)
Class EC 2.6.1.16
glutamine-fructose-6-phosphate transaminase (isomerizing)

Image of L-glutamine

Image of proton

Image of reduced ferredoxin

Image of 2-oxoglutarate

right arrow

Image of oxidised ferredoxin

Image of L-glutamate

L-glutamine
C00064
CHEBI:58359
2 proton
C00080
CHEBI:24636
2 reduced ferredoxin
C00138
CHEBI:17513
2-oxoglutarate
C00026
CHEBI:16810
2 oxidised ferredoxin
C00139
CHEBI:17908
2 L-glutamate
C00025
CHEBI:29985
0.4

Image of D-fructose 6-phosphate

Image of L-glutamine

right arrow

Image of L-glutamate

Image of D-glucosamine 6-phosphate

D-fructose 6-phosphate
C00085
CHEBI:61527
L-glutamine
C00064
CHEBI:32665
L-glutamate
C00025
CHEBI:16015
D-glucosamine 6-phosphate
C00352
CHEBI:57513

Catalytic CATH Codes

3.60.20.10
3.20.20.70

Catalytic CATH Codes

3.60.20.10
3.40.50.10490

Active Site



0.43315

Active Site



Catalytic Residues

Type Number Chain Location of Function
Cys 1 A Main Chain N Terminus
Side Chain
Arg 31 A Main Chain Carbonyl
Phe 207 A Main Chain Amide
Asn 227 A Side Chain
Gly 228 A Main Chain Amide
Glu 903 A Side Chain
Gln 969 A Side Chain
Lys 972 A Side Chain
Gln 978 A Main Chain Amide
0.4705

Catalytic Residues

Type Number Chain Location of Function
Cys 1 A Main Chain N Terminus
Side Chain
Arg 26 A Main Chain Carbonyl
Trp 74 A Main Chain Amide
Asn 98 A Side Chain
Gly 99 A Main Chain Amide
Glu 481 A Side Chain
Lys 485 A Side Chain
Glu 488 A Side Chain
Lys 603 A Side Chain
His 504 B Side Chain

Organic Cofactors

Type Identity Chain
FMN FMN 2508 A

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
iron F3S 2509 A

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 10 steps

0.808

Reaction occurs across 14 steps

Step 1
GIF of Reaction Step M0111.stg01

The N-terminus of Cys1 deprotonates deprotonates water, which deprotonates the thiol group of Cys1, initiating a nucleophilic attack on the amide carbon in an addition reaction.
1 Step 1
GIF of Reaction Step M0082.stg01

The N-terminus of Cys1 deprotonates water, which then deprotonates the thiol group of Cys1, initiating a nucleophilic attack on the amide carbon in an addition reaction.
Step 2
GIF of Reaction Step M0111.stg02

The oxyanion initiates an elimination that cleaves ammonia from the bound L-glutamine substrate. Ammonia deprotonates water, which deprotonates the N-terminus of Cys1.
1 Step 2
GIF of Reaction Step M0082.stg02

The oxyanion initiates an elimination that cleaves ammonia from the bound L-glutamine substrate. Ammonia deprotonates water, which deprotonates the N-terminus of Cys1.
Step 3
GIF of Reaction Step M0111.stg03

The N-terminus of Cys1 deprotonates water, which deprotonates another water that initiates a nucleophilic attack on the carbonyl carbon of the covalently bound intermediate in an addition reaction.
1 Step 3
GIF of Reaction Step M0082.stg03

The N-terminus of Cys1 deprotonates water, which initiates a nucleophilic attack on the carbonyl carbon of the covalently bound intermediate in an addition reaction.
Step 4
GIF of Reaction Step M0111.stg04

The oxyanion initiates an elimination that cleaves the C-S bond, the thiolate of Cys1 deprotonates water, which deprotonates the N-terminus of Cys1
1 Step 4
GIF of Reaction Step M0082.stg04

The oxyanion initiates an elimination that cleaves the C-S bond, the thiolate of Cys1 deprotonates water, which deprotonates the N-terminus of Cys1
Step 5
GIF of Reaction Step M0111.stg05

Ammonia initiates a nucleophilic attack on the C2 carbonyl carbon of the 2-oxoglutarate substrate in an addition reaction. The oxyanion formed deprotonates the bound ammonium.
0 Step 5
GIF of Reaction Step M0082.stg05

His504B deprotonates the hydroxyl group of C2 carbon in a decyclisation step.
Step 6
GIF of Reaction Step M0111.stg06

The amine initiates an elimination of the bound hydroxide as water, which deprotonates Lys972.
0.5 Step 6
GIF of Reaction Step M0082.stg06

The formed oxyanion deprotonates His504B
Step 7
GIF of Reaction Step M0111.stg07

FMN eliminates a hydride ion, which adds to the C2 imine carbon in an addition reaction.
0.09 Step 7
GIF of Reaction Step M0082.stg07

The C2 carbonyl oxygen deprotonates Lys603, initiating a nucleophilic attack of the Lys603 at C2 in an addition reaction.
Step 8
GIF of Reaction Step M0111.stg08

Lys972 deprotonates water in an inferred step.
0 Step 8
GIF of Reaction Step M0082.stg08

Lys603 initiates an elimination of water with concomitant deprotonation of itself.
Step 9
GIF of Reaction Step M0111.stg09

Ferredoxin donates a single electron, which is transferred to FMN through an iron-sulfur cluster. FMN deprotonates water.
0.4 Step 9
GIF of Reaction Step M0082.stg09

Ammonia initiates a nucleophilic attack on the C2 bound to Lys603 in an addition reaction with concomitant deprotonation of the ammonia by Lys603
Step 10
GIF of Reaction Step M0111.stg10

A second ferredoxin donates a single electron, which is transferred to FMN through an iron-sulfur cluster, which regenerates the reduced form of FMN.
0 Step 10
GIF of Reaction Step M0082.stg10

The newly bound amine eliminates Lys603 with concomitant deprotonation of itself by Lys603.
Step 11
No Step with this number present
N/A Step 11
GIF of Reaction Step M0082.stg11

Glu488 deprotonates the C1 with concomitant double bond rearrangement that deprotonates Lys485.
Step 12
No Step with this number present
N/A Step 12
GIF of Reaction Step M0082.stg12

Lys485 deprotonates the C1-OH with concomitant double bond rearrangement and deprotonation of Glu488 by the C2 carbon.
Step 13
No Step with this number present
N/A Step 13
GIF of Reaction Step M0082.stg13

His504B deprotonates the O5 hydroxyl.
Step 14
No Step with this number present
N/A Step 14
GIF of Reaction Step M0082.stg14

The oxyanion initiates a nucleophilic attack on the C1 carbonyl carbon in an addition reaction with concomitant deprotonation of His504B.

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