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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0102 and M0319

These two reactions have a combined similarity of 0.42


M0102

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Comparison

M0319

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EC 1.1.2.3
L-lactate dehydrogenase (cytochrome)
Sub-Class EC 1.6.99.1
NADPH dehydrogenase

Image of (S)-lactate

Image of ferricytochrome c

right arrow

Image of proton

Image of ferrocytochrome c

Image of pyruvate

(S)-lactate
C00186
CHEBI:16651
2 ferricytochrome c
C00125
CHEBI:16448
2 proton
C00080
CHEBI:24636
2 ferrocytochrome c
C00126
CHEBI:16707
pyruvate
C00022
CHEBI:15361
0.2

Image of NADPH

Image of proton

Image of a,b - unsaturated ketone

right arrow

Image of ketone

Image of NADP

NADPH
C00005
CHEBI:16474
proton
C00080
CHEBI:24636
a,b - unsaturated ketone
X00138
ketone
X00139
CHEBI:3992
NADP
C00006
CHEBI:18009

Catalytic CATH Codes

3.20.20.70

Catalytic CATH Codes

3.20.20.70

Active Site



0.2414

Active Site



Catalytic Residues

Type Number Chain Location of Function
Tyr 254 A Side Chain
Asp 282 A Side Chain
His 373 A Side Chain
0.25

Catalytic Residues

Type Number Chain Location of Function
His 191 A Side Chain
Asn 194 A Side Chain
Asn 251 A Side Chain
Tyr 196 A Side Chain
Thr 37 A Side Chain

Organic Cofactors

Type Identity Chain
FMN FMN 570 A

Organic Cofactors

Type Identity Chain
FMN FMN 401 A

Metal Cofactors

Type Het group Number Chain
iron HEM 560 A

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 6 steps

0.5227

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0102.stg01

His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.
0.52 Step 1
GIF of Reaction Step M0319.stg01

NADP reduces the FMN cofactor.
Step 2
GIF of Reaction Step M0102.stg02

A single electron is transferred from the FAD to the haem cofactor.
0.33 Step 2
GIF of Reaction Step M0319.stg02

The anionic FMN intermediate acts as a hydride donor to the a-b unsaturated substrate. Tyr196 acts as the proton donor in the trans reduction of the substrate double bond.
Step 3
GIF of Reaction Step M0102.stg03

The haem cofactor transfers the electron to ferricytochrome c.
0 Step 3
GIF of Reaction Step M0319.stg03

The Tyr196A is reprotonated by a solvent molecule.
Step 4
GIF of Reaction Step M0102.stg04

Water deprotonates the FAD, initiating the second single electron transfer from FAD to the haem cofactor.
N/A Step 4
No Step with this number present
Step 5
GIF of Reaction Step M0102.stg05

The haem cofactor transfers the electron to ferricytochrome c.
N/A Step 5
No Step with this number present
Step 6
GIF of Reaction Step M0102.stg06

Water deprotonates His373 in an inferred return step.
N/A Step 6
No Step with this number present

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