spacer

The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0102 and M0114

These two reactions have a combined similarity of 0.50


M0102

View
Comparison

M0114

View
EC 1.1.2.3
L-lactate dehydrogenase (cytochrome)
Sub-Class EC 1.5.8.2
trimethylamine dehydrogenase

Image of (S)-lactate

Image of ferricytochrome c

right arrow

Image of proton

Image of ferrocytochrome c

Image of pyruvate

(S)-lactate
C00186
CHEBI:16651
2 ferricytochrome c
C00125
CHEBI:16448
2 proton
C00080
CHEBI:24636
2 ferrocytochrome c
C00126
CHEBI:16707
pyruvate
C00022
CHEBI:15361
0.37

Image of electron-transferring flavoprotein

Image of trimethylamine

Image of water

right arrow

Image of proton

Image of reduced electron-transferring flavoprotein

Image of dimethylamine

Image of formaldehyde

electron-transferring flavoprotein
C04253
CHEBI:5086
trimethylamine
C00565
CHEBI:18139
water
C00001
CHEBI:15377
2 proton
C00080
CHEBI:24636
reduced electron-transferring flavoprotein
C04570
dimethylamine
C00543
CHEBI:17170
formaldehyde
C00067
CHEBI:16842

Catalytic CATH Codes

3.20.20.70

Catalytic CATH Codes

3.20.20.70

Active Site



0.45

Active Site



Catalytic Residues

Type Number Chain Location of Function
Tyr 254 A Side Chain
Asp 282 A Side Chain
His 373 A Side Chain
0.5

Catalytic Residues

Type Number Chain Location of Function
Tyr 169 A Side Chain
His 172 A Side Chain
Cys 30 A Side Chain

Organic Cofactors

Type Identity Chain
FMN FMN 570 A

Organic Cofactors

Type Identity Chain
FMN FMN 802 A

Metal Cofactors

Type Het group Number Chain
iron HEM 560 A

Metal Cofactors

Type Het group Number Chain
iron FS4 801 A

Reaction occurs across 6 steps

0.5526

Reaction occurs across 4 steps

Step 1
GIF of Reaction Step M0102.stg01

His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.
0.66 Step 1
GIF of Reaction Step M0114.stg01

The nitrogen of trimethylamine initiates the elimination of a hydride ion, which is added to FMN.
Step 2
GIF of Reaction Step M0102.stg02

A single electron is transferred from the FAD to the haem cofactor.
1 Step 2
GIF of Reaction Step M0114.stg02

The first single electron transfer from FMN to ETF, which passes through a single iron-sulfur cluster.
Step 3
GIF of Reaction Step M0102.stg03

The haem cofactor transfers the electron to ferricytochrome c.
0 Step 3
GIF of Reaction Step M0114.stg03

The second single electron transfer from FMN to ETF, which passes through a single iron-sulfur cluster. The transfer is facilitated by the abstraction of a proton from the FMN by water.
Step 4
GIF of Reaction Step M0102.stg04

Water deprotonates the FAD, initiating the second single electron transfer from FAD to the haem cofactor.
0 Step 4
GIF of Reaction Step M0114.stg04

The product of the enzyme undergoes spontaneous hydrolysis outside of the active site to produce formaldehyde and dimethylamine.
Step 5
GIF of Reaction Step M0102.stg05

The haem cofactor transfers the electron to ferricytochrome c.
N/A Step 5
No Step with this number present
Step 6
GIF of Reaction Step M0102.stg06

Water deprotonates His373 in an inferred return step.
N/A Step 6
No Step with this number present

spacer
spacer