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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0102 and M0109

These two reactions have a combined similarity of 0.37


M0102

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Comparison

M0109

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EC 1.1.2.3
L-lactate dehydrogenase (cytochrome)
Sub-Class EC 1.3.3.1
dihydroorotate oxidase

Image of (S)-lactate

Image of ferricytochrome c

right arrow

Image of proton

Image of ferrocytochrome c

Image of pyruvate

(S)-lactate
C00186
CHEBI:16651
2 ferricytochrome c
C00125
CHEBI:16448
2 proton
C00080
CHEBI:24636
2 ferrocytochrome c
C00126
CHEBI:16707
pyruvate
C00022
CHEBI:15361
0.08

Image of ubiquinone

Image of (S)-dihydroorotate

right arrow

Image of ubiquinol

Image of orotate

ubiquinone
C00399
CHEBI:16389
(S)-dihydroorotate
C00337
CHEBI:30864
ubiquinol
C00390
CHEBI:17976
orotate
C00295
CHEBI:30839

Catalytic CATH Codes

3.20.20.70

Catalytic CATH Codes

3.20.20.70

Active Site



0.0237

Active Site



Catalytic Residues

Type Number Chain Location of Function
Tyr 254 A Side Chain
Asp 282 A Side Chain
His 373 A Side Chain
0

Catalytic Residues

Type Number Chain Location of Function
Phe 149 A Side Chain
Ser 215 A Side Chain
Thr 218 A Side Chain
Lys 255 A Side Chain

Organic Cofactors

Type Identity Chain
FMN FMN 570 A

Organic Cofactors

Type Identity Chain
FMN FMN 398

Metal Cofactors

Type Het group Number Chain
iron HEM 560 A

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 6 steps

0.5531

Reaction occurs across 2 steps

Step 1
GIF of Reaction Step M0102.stg01

His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.
0.62 Step 1
GIF of Reaction Step M0109.stg01

Ser215 deprotonates the C5 of dihydroorotate, eliminating a hydride ion, which is added to FMN.
Step 2
GIF of Reaction Step M0102.stg02

A single electron is transferred from the FAD to the haem cofactor.
0.2 Step 2
GIF of Reaction Step M0109.stg02

The product dissociates from the active site, to be replaced by ubiquinone. The reduced FMN eliminates the hydride ion which is added to the ubiquinone, initiating double bond rearrangement and deprotonation of Ser215 to generate ubiquinol in an inferred return step.
Step 3
GIF of Reaction Step M0102.stg03

The haem cofactor transfers the electron to ferricytochrome c.
N/A Step 3
No Step with this number present
Step 4
GIF of Reaction Step M0102.stg04

Water deprotonates the FAD, initiating the second single electron transfer from FAD to the haem cofactor.
N/A Step 4
No Step with this number present
Step 5
GIF of Reaction Step M0102.stg05

The haem cofactor transfers the electron to ferricytochrome c.
N/A Step 5
No Step with this number present
Step 6
GIF of Reaction Step M0102.stg06

Water deprotonates His373 in an inferred return step.
N/A Step 6
No Step with this number present

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