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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0102 and M0108

These two reactions have a combined similarity of 0.30


M0102

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Comparison

M0108

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EC 1.1.2.3
L-lactate dehydrogenase (cytochrome)
Sub-Class EC 1.3.1.34
2,4-dienoyl-CoA reductase (NADPH)

Image of (S)-lactate

Image of ferricytochrome c

right arrow

Image of proton

Image of ferrocytochrome c

Image of pyruvate

(S)-lactate
C00186
CHEBI:16651
2 ferricytochrome c
C00125
CHEBI:16448
2 proton
C00080
CHEBI:24636
2 ferrocytochrome c
C00126
CHEBI:16707
pyruvate
C00022
CHEBI:15361
0.07

Image of NADPH

Image of trans,trans-2,3,4,5-tetradehydroacyl-CoA

Image of proton

right arrow

Image of NADP

Image of trans-2,3-didehydroacyl-CoA

NADPH
C00005
CHEBI:16474
trans,trans-2,3,4,5-tetradehydroacyl-CoA
C04512
CHEBI:58036
proton
C00080
CHEBI:24636
NADP
C00006
CHEBI:18009
trans-2,3-didehydroacyl-CoA
C00658
CHEBI:58856

Catalytic CATH Codes

3.20.20.70

Catalytic CATH Codes

3.20.20.70

Active Site



0.31627

Active Site



Catalytic Residues

Type Number Chain Location of Function
Tyr 254 A Side Chain
Asp 282 A Side Chain
His 373 A Side Chain
0.3333

Catalytic Residues

Type Number Chain Location of Function
Glu 164 A Side Chain
Tyr 166 A Side Chain
Arg 214 A Side Chain
His 252 A Side Chain
Gln 339 A Side Chain

Organic Cofactors

Type Identity Chain
FMN FMN 570 A

Organic Cofactors

Type Identity Chain
FAD FAD 701
FMN FMN 702

Metal Cofactors

Type Het group Number Chain
iron HEM 560 A

Metal Cofactors

Type Het group Number Chain
iron FS4 700 A

Reaction occurs across 6 steps

0.3425

Reaction occurs across 6 steps

Step 1
GIF of Reaction Step M0102.stg01

His373 deprotonates the alcohol of the lactate which eliminates a hydride ion that is added to FAD.
0.6 Step 1
GIF of Reaction Step M0108.stg01

NADP eliminates a hydride ion, which is added to FAD.
Step 2
GIF of Reaction Step M0102.stg02

A single electron is transferred from the FAD to the haem cofactor.
0.4 Step 2
GIF of Reaction Step M0108.stg02

FAD transfers a single electron, through Gln339 and an iron-sulfur cluster, to FMN, which deprotonates water.
Step 3
GIF of Reaction Step M0102.stg03

The haem cofactor transfers the electron to ferricytochrome c.
0 Step 3
GIF of Reaction Step M0108.stg03

Water deprotonates FAD, which initiates a second single electron transfer, through Gln339 and an iron-sulfur cluster, to FMN. This regenerates the FAD cofactor, and produces fully reduced FMN.
Step 4
GIF of Reaction Step M0102.stg04

Water deprotonates the FAD, initiating the second single electron transfer from FAD to the haem cofactor.
0.15 Step 4
GIF of Reaction Step M0108.stg04

FMN eliminates a hydride ion, which is added to the C5 of the substrate. This initiates double bond rearrangement, resulting in an oxyanion on the C1.
Step 5
GIF of Reaction Step M0102.stg05

The haem cofactor transfers the electron to ferricytochrome c.
0 Step 5
GIF of Reaction Step M0108.stg05

The oxyanion collapses, initiating double bond rearrangement, the C4 of the intermediate deprotonates Tyr166.
Step 6
GIF of Reaction Step M0102.stg06

Water deprotonates His373 in an inferred return step.
0.33 Step 6
GIF of Reaction Step M0108.stg06

Tyr166 deprotonates water in an inferred return step.

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