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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0050 and M0108

These two reactions have a combined similarity of 0.04


M0050

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Comparison

M0108

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EC 4.1.1.23
orotidine-5'-phosphate decarboxylase
Class EC 1.3.1.34
2,4-dienoyl-CoA reductase (NADPH)

Image of orotidine 5'-phosphate

Image of proton

right arrow

Image of carbon dioxide

Image of UMP

orotidine 5'-phosphate
C01103
CHEBI:57538
proton
C00080
CHEBI:24636
carbon dioxide
C00011
CHEBI:16526
UMP
C00105
CHEBI:57865
0.16

Image of NADPH

Image of trans,trans-2,3,4,5-tetradehydroacyl-CoA

Image of proton

right arrow

Image of NADP

Image of trans-2,3-didehydroacyl-CoA

NADPH
C00005
CHEBI:16474
trans,trans-2,3,4,5-tetradehydroacyl-CoA
C04512
CHEBI:58036
proton
C00080
CHEBI:24636
NADP
C00006
CHEBI:18009
trans-2,3-didehydroacyl-CoA
C00658
CHEBI:58856

Catalytic CATH Codes

3.20.20.70

Catalytic CATH Codes

3.20.20.70

Active Site



0.0118

Active Site



Catalytic Residues

Type Number Chain Location of Function
Asp 60 A Side Chain
Lys 62 A Side Chain
Asp 65 B Side Chain
0

Catalytic Residues

Type Number Chain Location of Function
Glu 164 A Side Chain
Tyr 166 A Side Chain
Arg 214 A Side Chain
His 252 A Side Chain
Gln 339 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

Type Identity Chain
FAD FAD 701
FMN FMN 702

Metal Cofactors

No Associated Metal Cofactors

Metal Cofactors

Type Het group Number Chain
iron FS4 700 A

Reaction occurs across 2 steps

0.035

Reaction occurs across 6 steps

Step 1
GIF of Reaction Step M0050.stg01

The substrate undergoes decarboxylation. The substrate concomitantly deprotonates Lys62 forming UMP.
0 Step 1
GIF of Reaction Step M0108.stg01

NADP eliminates a hydride ion, which is added to FAD.
Step 2
GIF of Reaction Step M0050.stg02

Lys62 deprotonates water.
0.4 Step 2
GIF of Reaction Step M0108.stg02

FAD transfers a single electron, through Gln339 and an iron-sulfur cluster, to FMN, which deprotonates water.
Step 3
No Step with this number present
N/A Step 3
GIF of Reaction Step M0108.stg03

Water deprotonates FAD, which initiates a second single electron transfer, through Gln339 and an iron-sulfur cluster, to FMN. This regenerates the FAD cofactor, and produces fully reduced FMN.
Step 4
No Step with this number present
N/A Step 4
GIF of Reaction Step M0108.stg04

FMN eliminates a hydride ion, which is added to the C5 of the substrate. This initiates double bond rearrangement, resulting in an oxyanion on the C1.
Step 5
No Step with this number present
N/A Step 5
GIF of Reaction Step M0108.stg05

The oxyanion collapses, initiating double bond rearrangement, the C4 of the intermediate deprotonates Tyr166.
Step 6
No Step with this number present
N/A Step 6
GIF of Reaction Step M0108.stg06

Tyr166 deprotonates water in an inferred return step.

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