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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0045 and M0285

These two reactions have a combined similarity of 0.95


M0045

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Comparison

M0285

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EC 2.4.1.19
cyclomaltodextrin glucanotransferase
Class EC 3.2.1.10
oligo-1,6-glucosidase

Image of Sugar

Image of 1,4-alpha-D-glucan chain

right arrow

Image of 1,4-alpha-D-glucan chain

Image of Sugar

Sugar
C11477
CHEBI:16646
1,4-alpha-D-glucan chain
C00718
CHEBI:15444
1,4-alpha-D-glucan chain
C00718
CHEBI:15444
Sugar
C11477
CHEBI:16646
1

Image of water

Image of isomaltose

right arrow

Image of alpha-D-glucose

Image of D-glucose

water
C00001
CHEBI:15377
isomaltose
C00252
CHEBI:28189
alpha-D-glucose
C00267
CHEBI:17634
D-glucose
C00031
CHEBI:17634

Catalytic CATH Codes

3.20.20.80

Catalytic CATH Codes

3.20.20.80

Active Site



0.82379

Active Site



Catalytic Residues

Type Number Chain Location of Function
Arg 227 A Side Chain
Asp 229 A Side Chain
Glu 257 A Side Chain
His 327 A Side Chain
Asp 328 A Side Chain
0.8571

Catalytic Residues

Type Number Chain Location of Function
Asp 199 A Side Chain
Glu 255 A Side Chain
Asp 325 A Side Chain
His 328 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

No Associated Metal Cofactors

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 2 steps

1

Reaction occurs across 2 steps

Step 1
GIF of Reaction Step M0045.stg01

Asp229 initiates a nucleophilic attack upon the carbon of the ether linkage between the two sugar rings in a substitution reaction, which eliminates a free sugar molecule, with concomitant deprotonation of Glu257.
1 Step 1
GIF of Reaction Step M0285.stg01

Asp displaces the terminal sugar in a nucleophilic substitution reaction, forming a covalent bond between the enzyme and substrate.
Step 2
GIF of Reaction Step M0045.stg02

Glu257 deprotonates a the free sugar molecule, which initiates a nucleophilic attack upon the covalently bound sugar in a substitution reaction, eliminating Asp229.
1 Step 2
GIF of Reaction Step M0285.stg02

Glu255 deprotonates the water, which initiates a nucleophilic attack on the covalently bound sugar, displacing the Asp199.

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