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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0035 and M0282

These two reactions have a combined similarity of 0.64


M0035

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Comparison

M0282

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EC 2.7.11.19
phosphorylase kinase
Sub-SubClass EC 2.7.12.2
mitogen-activated protein kinase kinase

Image of phosphorylase b

Image of ATP

right arrow

Image of ADP

Image of phosphorylase a

phosphorylase b
C02308
ATP
C00002
CHEBI:30616
ADP
C00008
CHEBI:456216
phosphorylase a
C02307
1

Image of serine protein

Image of ATP

right arrow

Image of phosphorylated serine protein

Image of ADP

serine protein
C02189
CHEBI:29999
ATP
C00002
CHEBI:30616
phosphorylated serine protein
X00134
CHEBI:61975
ADP
C00008
CHEBI:456216

Catalytic CATH Codes

1.10.510.10

Catalytic CATH Codes

1.10.510.10

Active Site



0.3792

Active Site



Catalytic Residues

Type Number Chain Location of Function
Asp 149 A Side Chain
Lys 151 A Side Chain
Thr 186 A Side Chain
0.4

Catalytic Residues

Type Number Chain Location of Function
Asp 194 A Side Chain
Asp 221 A Side Chain

Organic Cofactors

No Associated Organic Cofactors

Organic Cofactors

No Associated Organic Cofactors

Metal Cofactors

Type Het group Number Chain
magnesium MN 382 x
none MN 383 x

Metal Cofactors

Type Het group Number Chain
magnesium MG 536 A

Reaction occurs across 2 steps

0.7

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0035.stg01

Asp149 deprotonates the protein substrate hydroxyl group, which initiates a nucleophilic attack upon the gamma-phosphate of ATP in a substitution reaction that eliminates ADP.
0.25 Step 1
GIF of Reaction Step M0282.stg01

The serine residue of the protein substrate attacks the terminal, pentavalent phosphorous of ATP.
Step 2
GIF of Reaction Step M0035.stg02

The phospohrylated protein deprotonates Asp149.
1 Step 2
GIF of Reaction Step M0282.stg02

Asp194 abstracts a proton from the phospho-serine residue.
Step 3
No Step with this number present
N/A Step 3
GIF of Reaction Step M0282.stg03

Asp194 relays the proton to ADP, regenerating the active site.

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