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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0020 and M0294

These two reactions have a combined similarity of 0.51


M0020

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Comparison

M0294

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EC 1.3.99.1
succinate dehydrogenase
Sub-SubClass EC 1.3.5.1
succinate dehydrogenase (ubiquinone)

Image of acceptor

Image of proton

Image of fumarate

right arrow

Image of succinate

Image of reduced acceptor

acceptor
C00028
CHEBI:15339
2 proton
C00080
CHEBI:24636
fumarate
C00122
CHEBI:29806
succinate
C00042
CHEBI:30031
reduced acceptor
C00030
CHEBI:17499
0.62

Image of ubiquniol

Image of fumarate

right arrow

Image of ubiquinone

Image of succinate

ubiquniol
C00390
CHEBI:17976
fumarate
C00122
CHEBI:37154
ubiquinone
C00399
CHEBI:16389
succinate
C00042
CHEBI:30031

Catalytic CATH Codes

3.90.700.10
3.50.50.60
0.0.0.0

Catalytic CATH Codes

3.50.50.60
0.0.0.0

Active Site



0.68927

Active Site



Catalytic Residues

Type Number Chain Location of Function
His 365 A Side Chain
Glu 378 A Side Chain
Arg 381 A Side Chain
Arg 402 A Side Chain
His 504 A Side Chain
Arg 544 A Side Chain
0.7333

Catalytic Residues

Type Number Chain Location of Function
Phe 130 A Side Chain
Leu 263 A Side Chain
Glu 266 A Side Chain
Gln 251 A Side Chain
His 253 A Side Chain
His 364 A Side Chain
Arg 408 A Side Chain
Arg 297 A Side Chain

Organic Cofactors

Type Identity Chain
FAD FAD 805

Organic Cofactors

Type Identity Chain
FAD FAD 1001 A

Metal Cofactors

Type Het group Number Chain
iron HEC 801 _
iron HEC 802 _
iron HEC 803 _
iron HEC 804 _

Metal Cofactors

Type Het group Number Chain
iron SF4 1003 B
iron F3S 1004 B
iron No Available PDB Information

Reaction occurs across 3 steps

0.4382

Reaction occurs across 3 steps

Step 1
GIF of Reaction Step M0020.stg01

The electron acceptors donates two electrons, through a chain of haem cofactors to the FAD cofactor, which deprotonates a base, probably water.
0.38 Step 1
GIF of Reaction Step M0294.stg01

Ubiquinol acts as a two electron donor to the FAD cofactor, forming anionic FADH.
Step 2
GIF of Reaction Step M0020.stg02

FAD eliminates a hydride ion, which adds to fumarate with concomitant double bond rearrangement, the terminal carboxylate deprotonates His504.
0.55 Step 2
GIF of Reaction Step M0294.stg02

The FADH N5 acts as a hydride donor to the polarised double bond in fumarate. A proton is simultaneously abstracted from Arg297 to saturate the double bond, forming succinate.
Step 3
GIF of Reaction Step M0020.stg03

His504 deprotonates the newly formed hydroxyl group with concomitant deprotonation of Arg402, across the C=C, which initiates a proton relay chain through Glu378 and Arg381 to bulk solvent.
0.23 Step 3
GIF of Reaction Step M0294.stg03

The catalytic acid/base is reprotonated by a solvent molecule.

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