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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0020 and M0123

These two reactions have a combined similarity of 0.30


M0020

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Comparison

M0123

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EC 1.3.99.1
succinate dehydrogenase
Sub-Class EC 1.8.99.2
adenylyl-sulfate reductase

Image of acceptor

Image of proton

Image of fumarate

right arrow

Image of succinate

Image of reduced acceptor

acceptor
C00028
CHEBI:15339
2 proton
C00080
CHEBI:24636
fumarate
C00122
CHEBI:29806
succinate
C00042
CHEBI:30031
reduced acceptor
C00030
CHEBI:17499
0

Image of double-electron donor

Image of proton

Image of adenylylsulphate

right arrow

Image of AMP

Image of sulphite

Image of oxidised donor

double-electron donor
X00054
proton
C00080
CHEBI:24636
adenylylsulphate
C00224
CHEBI:58243
AMP
C00020
CHEBI:456215
sulphite
C00094
CHEBI:17137
oxidised donor
C02177

Catalytic CATH Codes

3.90.700.10
3.50.50.60
0.0.0.0

Catalytic CATH Codes

3.50.50.60
3.90.700.10
3.30.70.20

Active Site



0.30457

Active Site



Catalytic Residues

Type Number Chain Location of Function
His 365 A Side Chain
Glu 378 A Side Chain
Arg 381 A Side Chain
Arg 402 A Side Chain
His 504 A Side Chain
Arg 544 A Side Chain
0.3333

Catalytic Residues

Type Number Chain Location of Function
Asn 74 A Side Chain
Main Chain Amide
Glu 141 A Side Chain
Trp 234 A Side Chain
Arg 265 A Side Chain
Asp 361 A Side Chain
His 398 A Side Chain
Ser 449 A Side Chain
Main Chain Amide
Trp 48 B Side Chain

Organic Cofactors

Type Identity Chain
FAD FAD 805

Organic Cofactors

Type Identity Chain
FAD FAD 1000 A

Metal Cofactors

Type Het group Number Chain
iron HEC 801 _
iron HEC 802 _
iron HEC 803 _
iron HEC 804 _

Metal Cofactors

Type Het group Number Chain
iron SF4 1110 B
iron SF4 1100 B

Reaction occurs across 3 steps

0.3461

Reaction occurs across 5 steps

Step 1
GIF of Reaction Step M0020.stg01

The electron acceptors donates two electrons, through a chain of haem cofactors to the FAD cofactor, which deprotonates a base, probably water.
0.42 Step 1
GIF of Reaction Step M0123.stg01

A single electron is transferred from the donor, through two iron-sulfur clusters and Trp48B to FAD, with concomitant deprotonation of water.
Step 2
GIF of Reaction Step M0020.stg02

FAD eliminates a hydride ion, which adds to fumarate with concomitant double bond rearrangement, the terminal carboxylate deprotonates His504.
0.05 Step 2
GIF of Reaction Step M0123.stg02

A second single electron is transferred from the donor, through two iron-sulfur clusters and Trp48B to FAD forming the N1 anion form of reduced FAD.
Step 3
GIF of Reaction Step M0020.stg03

His504 deprotonates the newly formed hydroxyl group with concomitant deprotonation of Arg402, across the C=C, which initiates a proton relay chain through Glu378 and Arg381 to bulk solvent.
0 Step 3
GIF of Reaction Step M0123.stg03

The N5 of FAD initiates a nucleophilic attack on the sulfur of the sulfate group in an addition reaction.
Step 4
No Step with this number present
N/A Step 4
GIF of Reaction Step M0123.stg04

Water deprotonates the N5 of FAD. One of the oxygen anions of sulfate eliminates the phosphate group.
Step 5
No Step with this number present
N/A Step 5
GIF of Reaction Step M0123.stg05

The N5 of FAD initiates the elimination of the sulfite with concomitant deprotonation of water.

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