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The two reactions compared are done so using a Tanimoto similarity score (for more information, please see the MACiE FAQ) for the bond changes only. The score maay range from 0 to 1 where 1 indicates that the two reactions are identical at the bond change level and 0 indicates that there are no bond changes in common.


Key

1.0-0.9 0.9-0.8 0.8-0.7 0.7-0.6 0.6-0.5 0.5-0.4 0.4-0.3 0.3-0.2 0.2-0.1 0.1-0.0 =0

Results for Comparison of M0020 and M0006

These two reactions have a combined similarity of 0.31


M0020

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Comparison

M0006

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EC 1.3.99.1
succinate dehydrogenase
Sub-Class EC 1.8.1.7
glutathione-disulfide reductase

Image of acceptor

Image of proton

Image of fumarate

right arrow

Image of succinate

Image of reduced acceptor

acceptor
C00028
CHEBI:15339
2 proton
C00080
CHEBI:24636
fumarate
C00122
CHEBI:29806
succinate
C00042
CHEBI:30031
reduced acceptor
C00030
CHEBI:17499
0.07

Image of oxidised glutathione

Image of NADPH

Image of proton

right arrow

Image of glutathione

Image of NADP

oxidised glutathione
C00127
CHEBI:58297
NADPH
C00005
CHEBI:16474
proton
C00080
CHEBI:24636
2 glutathione
C00051
CHEBI:57925
NADP
C00006
CHEBI:18009

Catalytic CATH Codes

3.90.700.10
3.50.50.60
0.0.0.0

Catalytic CATH Codes

3.50.50.60
3.30.390.30

Active Site



0.18

Active Site



Catalytic Residues

Type Number Chain Location of Function
His 365 A Side Chain
Glu 378 A Side Chain
Arg 381 A Side Chain
Arg 402 A Side Chain
His 504 A Side Chain
Arg 544 A Side Chain
0.2

Catalytic Residues

Type Number Chain Location of Function
Cys 58 A Side Chain
Cys 63 A Side Chain
Lys 66 A Side Chain
Glu 201 A Side Chain
His 467 B Side Chain
Glu 472 B Side Chain

Organic Cofactors

Type Identity Chain
FAD FAD 805

Organic Cofactors

Type Identity Chain
FAD FAD 479
Glutathione GTT 0

Metal Cofactors

Type Het group Number Chain
iron HEC 801 _
iron HEC 802 _
iron HEC 803 _
iron HEC 804 _

Metal Cofactors

No Associated Metal Cofactors

Reaction occurs across 3 steps

0.3979

Reaction occurs across 6 steps

Step 1
GIF of Reaction Step M0020.stg01

The electron acceptors donates two electrons, through a chain of haem cofactors to the FAD cofactor, which deprotonates a base, probably water.
0.43 Step 1
GIF of Reaction Step M0006.stg01

NADP eliminates a hydride ion, which attacks the FAD cofactor with concomitant double bond rearrangement.
Step 2
GIF of Reaction Step M0020.stg02

FAD eliminates a hydride ion, which adds to fumarate with concomitant double bond rearrangement, the terminal carboxylate deprotonates His504.
0.22 Step 2
GIF of Reaction Step M0006.stg02

The FAD oxyanion collapses, initiating a nucleophilic attack on Cys63 in a substitution reaction that eliminates Cys58 from the disulfide bond.
Step 3
GIF of Reaction Step M0020.stg03

His504 deprotonates the newly formed hydroxyl group with concomitant deprotonation of Arg402, across the C=C, which initiates a proton relay chain through Glu378 and Arg381 to bulk solvent.
0.06 Step 3
GIF of Reaction Step M0006.stg03

The FAD cofactor eliminates Cys63 with concomitant deprotonation of the nitrogen to which the hydride was added.
Step 4
No Step with this number present
N/A Step 4
GIF of Reaction Step M0006.stg04

His467B deprotonates Cys63.
Step 5
No Step with this number present
N/A Step 5
GIF of Reaction Step M0006.stg05

Cys58 initiates a nucleophilic attack on the disulfide bond of oxidised glutathione in a substitution reaction, eliminating glutathione with concomitant deprotonation of His467B.
Step 6
No Step with this number present
N/A Step 6
GIF of Reaction Step M0006.stg06

Cys63 initiates a nucleophilic attack on Cys58 in a substitution reaction, eliminating glutathione with concomitant deprotonation of water.

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