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Entry M0347    4.1.-.-    feruloyl CoA hydratase/lyase

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Step 02

The quinone-methide-enolate is the substrate for hydration by a water molecule activated by Glu143.

GIF of Reaction Step M0347.stg02



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Reactant Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Met70A Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Gly120A Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Tyr75A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Steric Role
Tyr239B Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Steric Role

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
O-H
O-H
The C-C bond changes from a single to double bond
The C-C bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-C bond changes from a double to single bond
The C-O bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


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