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Overview for MACiE Entry M0344

Version history

General Information

EC Number: 2.3.1.48 (A member of the Transferases, Acyltransferases, Transferring groups other than aminoacyl groups)

Enzyme Name: histone acetyltransferase

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • Q9Y232 - Chromodomain Y-like protein

Representative PDB Code: 2gtr - HUMAN CHROMODOMAIN Y-LIKE PROTEIN (Resolution = 1.90 Å).

Catalytic CATH Codes:

  • Unassigned Domain

Display structure information

Overall Reaction:

Image of acetyl-CoA

Image of histone-L-lysine

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Image of CoA

Image of histone N6-acetyl-lysine

acetyl-CoA
C00024
CHEBI:57288
histone-L-lysine
C02415
CoA
C00010
CHEBI:57287
histone N6-acetyl-lysine
C01997

Overall Comment: Enzymes in the histone acetyltransferase family of the crotonase superfamily catalyse the acetylation of N-terminal lysine residues in histones, as part of the displacement of histones by transition proteins and protamines during vertebrate spermatogenesis


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Stepwise Description of the Reaction

Step 1Asp146 abstracts a proton from the NE group of lysine in histones, with the concomitant attack of the amino group to C1 in the substrate. This is facilitated by the binding of the thioester oxygen atom to an oxyanion hole formed by the peptide nitrogens of Leu66 and of Leu115.
Step 2The oxyanion intermediate collapses to give two products with the help of a proton donated by Asp146

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Leu 66 A Main Chain Amide
Leu 115 A Main Chain Amide
Asp 146 A Side Chain

References

  1. B.T. Lahn et al. (2002), Proc .Natl Acad. Sci .USA, 99, 8707-8712. Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis.
    Medline: 12072557

Homologue information for M0344 (2gtr)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with the same EC number (different mechanisms):

MACiE Entry Enzyme Name PDB code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0224 histone acetyltransferase 1bob 0.6666 0.0318 Compare

Compare of the other reactions in MACiE with EC number 2.3.1.48


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

GOA logo
catalytic activity (molecular function)
histone acetyltransferase activity (molecular function)
nucleus (cellular component)
regulation of transcription, DNA-templated (biological process)
spermatogenesis (biological process)
metabolic process (biological process)
transferase activity (molecular function)
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