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Entry M0341    5.3.3.8    dodecenoyl-CoA isomerase

Previous Step

Step 02

The oxyanion intermediate tautomerises to give product with the help of a proton donated by Glu136

GIF of Reaction Step M0341.stg02


Comment: In PDB 1sg4, Glu136 OE2 atom is 3.7 Angstoms away from C2 of the substrate, and 4.1 Angstroms away from C3 and C4 [1].



Mechanisms

Proton Transfer
Assisted Keto-Enol Tautomerisation

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Terminated
Overall Product Formed
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Leu66A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Glu136A Side Chain reactant Polar Non-polar interaction
Proton Donor
Gly111A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
O-H
The C-C bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-C bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


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