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Entry M0327    2.7.3.-    protein histidine kinase (CheA)

Next Step

Step 01

His45, activated by Glu67, acts as a nucleophile towards the terminal phosphate group of ATP.

GIF of Reaction Step M0327.stg01


Comment: The protonation state of the ATP and transferred phosphate is not specified.



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Intermediate Formation
Overall Product Formed
Overall Reactant Used
Enzyme-Substrate Bond Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asn409 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Steric Role
His45 Side Chain reactant Activator
Nucleophile
Hydrogen Bond Donor
Covalently Attached
Glu67 Side Chain spectator Electrostatic Stabiliser
Increase Nucleophilicity

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG 382 A spectator Substrate Binding
Increase Electrophilicity
Activator
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-N
P-O
None
N
O
P

View similar reactions in MACiE.


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