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Entry M0326    2.7.1.40    pyruvate kinase

Next Step

Step 01

Phosphoryl transfer from PEP to M(II)ADP occurs by an apparent SN2 mechanism with an inversion of configuration at the phosphoryl group, to yield the enolate of pyruvate and M(II)ATP

GIF of Reaction Step M0326.stg01


Comment: The presence of either a Mn or Mg divalent cation is essential for catalytic activity [4]. The monovalent cation, K+, does not directly contact the substrate or intermediate, but instead is thought to influence the structure of the active site though interactions with the positively charged resides Arg72, Arg119 and Lys269 [4].



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Overall Reactant Used
Overall Product Formed
Intermediate Formation
Dephosphorylation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg72 Side Chain spectator Attractive Charge Charge Interaction
Electrostatic Stabiliser
Lys269 Side Chain spectator Attractive Charge Charge Interaction
Hydrogen Bond Donor
Electrostatic Stabiliser
Steric Role
Arg119 Side Chain spectator Attractive Charge Charge Interaction
Electrostatic Stabiliser
Thr327 Side Chain spectator Hydrogen Bond Donor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
potassium K 3532 E spectator Not Active
manganese MG 3534 E spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity
none MG 3536 E spectator Substrate Binding
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
P-O
None
O
P

View similar reactions in MACiE.


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