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Entry M0320    1.8.5.-    sulfide:quinone oxidoreductase

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Step 08

A second molecule of sulfide enters the active site and acts as a nucleophile towards the three member sulfur bridge between Cys178A and Cys350A.

GIF of Reaction Step M0320.stg08



Mechanisms

Bimolecular Nucleophilic Substitution
Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Enzyme-Substrate Bond Formation
Overall Reactant Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp215A Side Chain reactant Activator
Proton Acceptor
Cys350A Side Chain reactant Activator
Electrophile
Covalently Attached
Cys178A Side Chain reactant Activator
Nucleofuge
Asp353A Side Chain reactant Activator
Proton Donor
Proton Acceptor
Lys386A Side Chain spectator Hydrogen Bond Donor
Cys129A Side Chain reactant Activator
Covalently Attached
Nucleofuge

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD401A spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
S-S
O-H
S-H
S-S
The C-O bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
O
S

View similar reactions in MACiE.


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