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Entry M0320    1.8.5.-    sulfide:quinone oxidoreductase

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Step 02

The anionic Cys178A attacks the C4 position of FAD, initiating protonation of the N5 position.

GIF of Reaction Step M0320.stg02



Mechanisms

Bimolecular Nucleophilic Addition
Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Cofactor Used
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp215A Side Chain spectator Hydrogen Bond Donor
Cys350A Side Chain reactant Activator
Covalently Attached
Cys178A Side Chain reactant Activator
Nucleophile
Asp353A Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Cys129A Side Chain spectator Covalently Attached

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD401A reactant Activator
Covalently Attached
Polar Interaction
Electrophile
Proton Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-S
S-H
The C-N bond changes from a double to single bond
C
H
N
S

View similar reactions in MACiE.


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