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Entry M0317    6.3.2.9    UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase

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Step 04

The tetrahedral intermediate collapses, releasing a peptidoglycan molecule and protonated phosphate.

GIF of Reaction Step M0317.stg04



Mechanisms

Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys115A Side Chain spectator Hydrogen Bond Donor
Asn138A Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
His183A Side Chain spectator Hydrogen Bond Donor

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
unknown MG 1000 A spectator Activator
Substrate Binding
unknown MG 1001 A spectator Not Present

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
C-O
The C-O bond changes from a single to double bond
C
O

View similar reactions in MACiE.


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