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Entry M0301    3.5.1.2    glutaminase

Previous Step

Step 04

The tetrahedral intermediate collapses, cleaving the acyl-enzyme bond and liberating Ser74, which in turn deprotonates the Lys77 via a proton relay with Tyr201.

GIF of Reaction Step M0301.stg04



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Enzyme Regenerated
Intermediate Collapse
Intermediate Terminated
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Val271 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser74A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr253 Side Chain spectator Hydrogen Bond Acceptor
Tyr201 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Acceptor
Proton Donor
Proton Relay
Ser74A Side Chain reactant Covalently Attached
Nucleofuge
Proton Acceptor
Hydrogen Bond Acceptor
Lys77 Side Chain reactant Hydrogen Bond Donor
Proton Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
C-O
N-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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