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Entry M0301    3.5.1.2    glutaminase

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Step 03

Lys77 deprotonates water via a proton relay with Tyr201, which initiates a nucleophilic addition at the carbonyl carbon, forming a new tetrahedral intermediate.

GIF of Reaction Step M0301.stg03



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Reactant Used
Enzyme-Substrate Bond Formation
Intermediate Formation
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Val271 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser74A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr253 Side Chain spectator Hydrogen Bond Acceptor
Tyr201 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Acceptor
Proton Donor
Proton Relay
Ser74A Side Chain reactant Covalently Attached
Activator
Lys77 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
O-H
O-H
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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