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Entry M0301    3.5.1.2    glutaminase

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Step 02

The tetrahedral intermediate collapses, cleaving the C-N bond and releasing the ammonia product, the nitrogen of which deprotonates Lys77 via a proton relay through Tyr253.

GIF of Reaction Step M0301.stg02



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Intermediate Formation
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Val271 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ser74A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr253 Side Chain reactant Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Donor
Proton Acceptor
Proton Relay
Tyr201 Side Chain spectator Hydrogen Bond Acceptor
Ser74A Side Chain reactant Covalently Attached
Activator
Lys77 Side Chain reactant Hydrogen Bond Donor
Proton Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
C-N
N-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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