spacer

Entry M0284    3.5.5.8    thiocyanate hydrolase

Previous Step

Step 07

An intermolecular elimination of hydroxide occurs, forming carbonyl sulfide.

GIF of Reaction Step M0284.stg07



Mechanisms

Unimolecular Elimination by the Conjugate Base

Mechanism Components

Enzyme Regenerated
Bond Cleavage
Bond Order Change
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser132C Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Activator
Ser132C Main Chain Amide spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cys128C Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cso133C Main Chain Amide spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cso133C Post-Translationally Modified Residue spectator Metal Ligand
Electrostatic Stabiliser
Tyr108C Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Csd131C Post-Translationally Modified Residue spectator Metal Ligand
Electrostatic Stabiliser
Activator

Metal Cofactors involved in Step 07

Metal Type Metal Identity Chain Activity Function
cobalt 3CO 301 C spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
C-O
The C-S bond changes from a single to double bond
C
O
S

View similar reactions in MACiE.


spacer
spacer