Entry M0284 126.96.36.199 thiocyanate hydrolase
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The hydroxyl present on Cys133 activates a water molecule to act as a nucleophile towards the thio-acid intermediate.
Comment: The post translation modified hydroxyl group at Cys133 is thought to behave as a general base towards the incoming water molecule.
The enzyme is only active once hydroxylation has occurred at this position, and the existence of such a post translational modification has been hypothesized as a regulatory mechanism for enzyme activity .
Amino acids involved in the reaction step.
Metal Cofactors involved in Step 05
||Bonds Changed in Order
||Atom Types Involved
The C-O bond changes from a double to single bond
View similar reactions in MACiE.