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Entry M0284    3.5.5.8    thiocyanate hydrolase

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Step 03

Tautomerisation of the intermediate results in a carbonyl species.

This reaction step has been inferred

GIF of Reaction Step M0284.stg03



Mechanisms

Keto-Enol Tautomerisation

Mechanism Components

Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser132C Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Activator
Ser132C Main Chain Amide spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cys128C Side Chain spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cso133C Main Chain Amide spectator Metal Ligand
Electrostatic Stabiliser
Activator
Cso133C Post-Translationally Modified Residue spectator Metal Ligand
Electrostatic Stabiliser
Activator
Tyr108C Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Csd131C Post-Translationally Modified Residue spectator Metal Ligand
Electrostatic Stabiliser
Activator

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
cobalt 3CO 301 C spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
The C-O bond changes from a single to double bond
The C-N bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


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