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Entry M0281    1.5.5.1    electron-transferring-flavoprotein dehydrogenase

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Step 04

The semiquinone FAD intermediate reduces the bound ubiquinone substrate.

GIF of Reaction Step M0281.stg04


Comment: Ubiquinone structures where n=5-9 are known substrates.



Mechanisms

Electron Transfer
Proton Transfer
Redox

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Overall Reactant Used
Cofactor Regenerated
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg331A Side Chain spectator Hydrogen Bond Donor
Arg331A Main Chain Carbonyl spectator Hydrogen Bond Acceptor
Cys556A Side Chain spectator Activator
Metal Ligand
Attractive Charge Charge Interaction
Electrostatic Stabiliser
Cys556A Main Chain Carbonyl spectator Not Active
Cys527A Side Chain spectator Activator
Metal Ligand
Attractive Charge Charge Interaction
Electrostatic Stabiliser
Thr367B Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys559A Side Chain spectator Activator
Metal Ligand
Attractive Charge Charge Interaction
Electrostatic Stabiliser
Cys553A Side Chain spectator Activator
Metal Ligand
Attractive Charge Charge Interaction
Electrostatic Stabiliser
Ser82A Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD611A reactant Activator
One Electron Donor
Proton Donor
Hydrogen Bond Acceptor

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
iron SF4 610 A spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
N-H
The C-N bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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