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Entry M0281    1.5.5.1    electron-transferring-flavoprotein dehydrogenase

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Step 03

The one electron oxidised electron transfer flavoprotein donates a second electron to the iron sulfur cluster, reducing its overall charge from +2 to +1.

GIF of Reaction Step M0281.stg03


Comment: On the addition of a second electron from the ET-transport flavin, both the flavin cofactor and Fe-S cluster are in a single electron reduced states, i.e a semi-quinone and +1 state, respecitively [1].



Mechanisms

Electron Transfer
Redox

Mechanism Components

Cofactor Used
Intermediate Formation
Bond Order Change

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg331A Side Chain spectator Hydrogen Bond Donor
Arg331A Main Chain Carbonyl spectator Hydrogen Bond Acceptor
Cys556A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Electrostatic Stabiliser
Cys556A Main Chain Carbonyl spectator Not Active
Cys527A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Electrostatic Stabiliser
Thr367B Side Chain spectator Hydrogen Bond Donor
Cys559A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Electrostatic Stabiliser
Cys553A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Electrostatic Stabiliser
Ser82A Side Chain spectator Hydrogen Bond Donor

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD611A spectator Hydrogen Bond Acceptor

Metal Cofactors involved in Step 03

Metal Type Metal Identity Chain Activity Function
iron SF4 610 A reactant One Electron Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
The C-N bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


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