spacer

Entry M0281    1.5.5.1    electron-transferring-flavoprotein dehydrogenase

Next Step

Step 01

The reduced electron transfer flavoprotein delivers electron to the FAD cofactor via the iron sulfur cluster.

GIF of Reaction Step M0281.stg01


Comment: Mutagenesis and kinetic calculations have shown the electron to relay through the Fe4S4 cluster before reaching the FAD cofactor, rather than being directly donated to the flavin [2]. The electron is relayed to FAD from the iron sulfur cluster through interactions between the close proximity cabronyl of the metal ligand Cys556 and the carbonyl of Arg331 [1].



Mechanisms

Electron Transfer
Redox

Mechanism Components

Bond Order Change
Overall Reactant Used
Cofactor Used
Intermediate Formation
Electron Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg331A Side Chain reactant Activator
Electron Relay
One Electron Acceptor
One Electron Donor
Hydrogen Bond Donor
Arg331A Main Chain Carbonyl reactant Activator
Electron Relay
One Electron Acceptor
One Electron Donor
Hydrogen Bond Acceptor
Cys556A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Cys556A Main Chain Carbonyl reactant Activator
One Electron Acceptor
One Electron Donor
Electron Relay
Hydrogen Bond Acceptor
Cys527A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Thr367B Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Cys559A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Cys553A Side Chain spectator Activator
Attractive Charge Charge Interaction
Metal Ligand
Ser82A Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Organic Cofactors involved in the reaction step

Cofactor Type Cofactor Activity Function
FAD FAD611A reactant Activator
One Electron Acceptor
Hydrogen Bond Acceptor

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
iron SF4 610 A reactant Electron Relay
One Electron Donor
One Electron Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
None
The C-C bond changes from a single to double bond
The C-N bond changes from a double to single bond
The C-O bond changes from a double to single bond
C
N
O

View similar reactions in MACiE.


spacer
spacer